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Chinese Journal of Virology ; (6): 522-528, 2013.
Artículo en Chino | WPRIM | ID: wpr-356672

RESUMEN

To reveal the genetic variation of the viral protein 1 (VP1) gene of the duck hepatitis A virus type 3 (DHAV-3), the VP1 gene of 13 virulent DHAV-3 strains isolated from Shandong province of China in 2012 were amplified by RT-PCR, sequenced and analyzed. The results showed that all the VP1 genes of the 13 isolates contained 720 nucleotides encoding 240 amino acids, and shared with nucleotide identities of 94. 6%-99.9% and amino acid identities of 95.0%-100%. The nucleotide and amino acid sequence homologies between the 13 DHAV-3 isolates and other 31 DHAV-3 reference strains were 92.5%-100% and 90. 8%-100%, respectively. Phylogenetic analysis showed that the VP1 gene of DHAV-3 had distinct geographical characteristics. Distribution of genotypes of the 44 DHAV-3 strains was as follows: except the vaccine strain B63, all the other Chinese isolates belonged to genotype I (GI), Vietnamese wild isolates mainly belonged to subtype 1 (S1) of genotype II (GII), and all Korean isolates belonged to subtype 2 (S2) of GII.


Asunto(s)
Animales , Secuencia de Aminoácidos , Proteínas de la Cápside , Química , Genética , China , Patos , Virus de la Hepatitis del Pato , Clasificación , Genética , Hepatitis Viral Animal , Virología , Datos de Secuencia Molecular , Filogenia , Infecciones por Picornaviridae , Virología , Enfermedades de las Aves de Corral , Virología
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