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Objective To analyze the clinical effect of neoadjuvant chemotherapy( oxaliplatin combined with S-1) in the treatment of advanced gastric cancer,and to detect its influence on expressions of vascular endothelial growth factor(VEGF) and matrix metalloproteinase-2(MMP-2).Methods A total of 70 patients with advanced gastric cancer were randomly divided into research group ( n=35 ) and control group ( n=35 ) .The patients in the control group were given surgery treatment and general drug chemotherapy,while the patients in the research group were given the neoadjuvant chemotherapy on the basis of the control group.The incidence of cancer recurrence and the expressions of MMP-2,VEGF of the two groups were observed.Results In the research group,the expressions of MMP-2 or VEGF were significantly different before and after chemotherapy(χ2 =10.03,χ2 =9.11,all P<0.01). The 3-year survival rate in the research group(71.43%) was significantly higher than 45.71% in the control group,there was significant difference(χ2 =11.70,P<0.05).Conclusion Neoadjuvant chemotherapy can reduce the expressions of MMP-2 and VEGF in tumor tissues,indicates that it may play an important role in patients with advanced gastric cancer and can be used in clinic.
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Objective To investigate the electrophysiological properties of layer Ⅱ/Ⅲ pyramidal neurons of the early postnatal stage rat visual cortex.Methods By using the whole cell patch-clamp recording technique combined with direct visualization of cells,we studied the passive and active electrophysiological properties as well as firing properties of layer Ⅱ/Ⅲ pyramidal neurons in acute rat visual cortical slices.Results Resting membrane potential,input resistance,membrane capacitance and membrane time constant of pyramidal neurons at postnatal days 11-13(P 11-13) were(-56.6?1.8)mV,(185.4?2.7)M?,(77.9?2.2)pF and(16.9?2.4)ms,respectively.Action potential amplitude and duration were(97.7?2.7)mV and(2.3?0.1)ms,respectively.Threshold potential was(-31.8?1.2)mV and afterdepolarizing potential was(-65.3?1.1)mV.When presented with long depolarizing current pulse of constant amplitude,most of the neurons exhibited pronounced adaptation of spike frequency.The steady-state firing frequency was(30.4?9.4)% of the first interval firing frequency.Conclusion The electrophysiological properties of layer Ⅱ/Ⅲ pyramidal neurons of the early postnatal stage rat visual cortex are not fully mature.Most of the neurons display regular firing patterns,but the degree of firing frequency adaptation is relatively small.