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Precision medicine became the key strategy in development priority of science and technology in China.The large population-based cohorts become valuable resources in preventing and treating major diseases in the population,which can contribute scientific evidence for personalized treatment and precise prevention.The fundamental question of the achievements above,therefore,is how to construct a large population-based cohort in a standardized way.The Chinese Preventive Medicine Association co-ordinated experienced researchers from Peking University and other well-known institutes to write up two group standards Technical specification of data processing for large population-based cohort study (T/CPMA 001-2018) and Technical specification of data security for large population-based cohort study (T/CPMA 002-2018),on data management.The standards are drafted with principles of emphasizing their scientific,normative,feasible,and generalizable nature.In these two standards,the key principles are proposed,and technical specifications are recommended in data standardization,cleansing,quality control,data integration,data privacy protection,and database security and stability management in large cohort studies.The standards aim to guide the large population-based cohorts that have been or intended to be established in China,including national cohorts,regional population cohorts,and special population cohorts,hence,to improve domestic scientific research level and the international influence,and to support decision-making and practice of disease prevention and control.
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Precision medicine became the key strategy in development priority of science and technology in China.The large population-based cohorts become valuable resources in preventing and treating major diseases in the population,which can contribute scientific evidence for personalized treatment and precise prevention.The fundamental question of the achievements above,therefore,is how to construct a large population-based cohort in a standardized way.The Chinese Preventive Medicine Association co-ordinated experienced researchers from Peking University and other well-known institutes to write up two group standards Technical specification of data processing for large population-based cohort study (T/CPMA 001-2018) and Technical specification of data security for large population-based cohort study (T/CPMA 002-2018),on data management.The standards are drafted with principles of emphasizing their scientific,normative,feasible,and generalizable nature.In these two standards,the key principles are proposed,and technical specifications are recommended in data standardization,cleansing,quality control,data integration,data privacy protection,and database security and stability management in large cohort studies.The standards aim to guide the large population-based cohorts that have been or intended to be established in China,including national cohorts,regional population cohorts,and special population cohorts,hence,to improve domestic scientific research level and the international influence,and to support decision-making and practice of disease prevention and control.
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Objective The study was to investigate the activation of tumor necrosis factor α (TNF-α) mRNA transcription and protein expression in peripheral blood and activation of signal path PI3K/AKT in patients with chronic heart failure. Methods From February 2015 to April 2018, 244 patients with heart failure in the cardiovascular department of our hospital were selected as heart failure group, while 244 healthy cases were enrolled as the control group at the same time. The peripheral blood samples of two groups were collected. We detected the transcription and protein expression of TNF-α mRNA and the activation of PI3K, AKT in peripheral blood. The left ventricular ejection fraction (LVEF) were measured in two groups. The correlations between influencing factors and LVEF were analyzed. Results The levels of PI3K, AKT in the heart failure group were higher than those in the control group. The differences were statistically significant respectively (P < 0.05). The mRNA relative content and protein content of TNF-α in peripheral blood mononuclear cells of heart failure group were higher compared with those of control group (P < 0.05). The LVEF of heart failure group was significantly lower than that of the control group (34.50 ± 6.33) ％ versus (55.60 ± 2.49) ％, P < 0.001). Among 244 patients with heart failure, Spearman correlation analysis showed that there were significant positive correlations between TNF-a mRNA and protein expression levels and the levels of PI3K, AKT respectively (P < 0.05). Multiple factors unconditional Logistic regression analysis showed that the TNF-α mRNA, protein expression and PI3K, AKT levels in peripheral blood were independent risk factors for LVEF (P < 0.05). Conclusion The expression levels of PI3K, AKT and TNF-α are all significantly increased in chronic heart failure patients, which could participate in the occurrence and development of heart failure.
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Large population-based cohort study is an important resource for population disease prevention and control, the results of which provide scientific basis for individualized treatment and precise prevention, and it is also the key strategic content of precision medicine. The Chinese Preventive Medicine Association coordinated experienced researchers from the Chinese Academy of Medical Sciences and other professional institutes to write up the group standard entitled Technical specification of management for field investigation in large population-based cohort study (T/CPMA 001-2019). Based on the research of large population-based cohort study in China and the principle of scientific, normative, applicable, and feasible, the standard proposed six aspects of management requirements including institutional arrangement, personnel composition, equipment, materials, documents and finance, as well as the basic requirements of the on-site investigation, the requirements of each position and the overall quality control requirements, etc.. The standard aims to guide the large population-based cohorts that have been or intended to be established in China, including national cohorts, regional population cohorts, and special population cohorts, hence to improve scientific research level, accelerate scientific research output and provide localization basis for disease prevention and control in China.
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Objective To explore the relationship of family environment in childhood and adolescence and mental health in adulthood.Methods A total of 791 subjects aged ≥25 years were selected through the Chinese National Twin Registry (CNTR).The short-form of Family Environment Scale-Chinese Version (FES-CV) was used to evaluate the family environment during childhood and adolescence in three dimensions:relationship,system maintenance and personal growth.The mental health status in adulthood was assessed with the Chinese version of 6-item Kessler Psychological Distress Scale (K6).The generalized linear mixed model was used to examine their relationship.Results About 4.6％ of the subjects reported general or worse mental health status.Compared with the subjects with good mental status,statistical difference was observed only in parenting way among twins (living together or not).After adjusting the potential confounders,such as age,sex,zygosity,education and lifestyle (smoking,drinking and physical activity),good family relationship and system maintenance had a positive effect on mental health,with the OR (95％CI) of 0.66 (0.5 1-0.87) and 0.70 (0.50-0.98) respectively.Furthermore,parenting way did not modify the effect of family environment on mental health status in adulthood (interaction:P＞0.05).In each scale,scores of cohesion and organization were positively correlated with mental health,while the score of conflict was negatively correlated with the mental health.Conclusion Good family relationship and system maintenance in childhood and adolescence had a positive impact on mental health in adulthood.
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Objective To explore the relationship of family environment in childhood and adolescence and mental health in adulthood.Methods A total of 791 subjects aged ≥25 years were selected through the Chinese National Twin Registry (CNTR).The short-form of Family Environment Scale-Chinese Version (FES-CV) was used to evaluate the family environment during childhood and adolescence in three dimensions:relationship,system maintenance and personal growth.The mental health status in adulthood was assessed with the Chinese version of 6-item Kessler Psychological Distress Scale (K6).The generalized linear mixed model was used to examine their relationship.Results About 4.6％ of the subjects reported general or worse mental health status.Compared with the subjects with good mental status,statistical difference was observed only in parenting way among twins (living together or not).After adjusting the potential confounders,such as age,sex,zygosity,education and lifestyle (smoking,drinking and physical activity),good family relationship and system maintenance had a positive effect on mental health,with the OR (95％CI) of 0.66 (0.5 1-0.87) and 0.70 (0.50-0.98) respectively.Furthermore,parenting way did not modify the effect of family environment on mental health status in adulthood (interaction:P＞0.05).In each scale,scores of cohesion and organization were positively correlated with mental health,while the score of conflict was negatively correlated with the mental health.Conclusion Good family relationship and system maintenance in childhood and adolescence had a positive impact on mental health in adulthood.
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Objective To Investigate the immune status and influencing factors of provincial polio network laboratory (PNL) workers in China so as to provide evidence for the development of related strategies to protect personnel working at the PNLs.Methods All the practitioners from the PNLs at the provincial centers for disease control,were selected as objects for this study,from October to December,2016,under a questionnaire survey.Information on status of immunity and influencing factors was collected,with SAS software,trend chi-square used for statistics analysis.Results A total of 77 workers were involved in this survey,with 60 (78％) of them completed the polio-based immune program but the rest 17 (22％) remained records unclear.66 people (about 86％) remembered clearly that they had received vaccination when engaging in the polio-lab work,but the rest 11 (14％) with only partial vaccination records.We also noticed that the Influencing factors realted to vaccination status were:age (x2 =2.48,P＜0.05),title (x2 =2.51,P＜0.05),years of employment (P＜0.000 1),education (x2 =0.74,P=0.46) and gender (x2 =0.46,P=0.50).Conclusion Immune status of the Chinese provincial PNL practitioners appeared fairly good as 86％ of all the workers had received polio-related vaccination,with 41％ of them completed a 3-time inoculation program,when started working in this field.
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Objective To Investigate the immune status and influencing factors of provincial polio network laboratory (PNL) workers in China so as to provide evidence for the development of related strategies to protect personnel working at the PNLs.Methods All the practitioners from the PNLs at the provincial centers for disease control,were selected as objects for this study,from October to December,2016,under a questionnaire survey.Information on status of immunity and influencing factors was collected,with SAS software,trend chi-square used for statistics analysis.Results A total of 77 workers were involved in this survey,with 60 (78％) of them completed the polio-based immune program but the rest 17 (22％) remained records unclear.66 people (about 86％) remembered clearly that they had received vaccination when engaging in the polio-lab work,but the rest 11 (14％) with only partial vaccination records.We also noticed that the Influencing factors realted to vaccination status were:age (x2 =2.48,P＜0.05),title (x2 =2.51,P＜0.05),years of employment (P＜0.000 1),education (x2 =0.74,P=0.46) and gender (x2 =0.46,P=0.50).Conclusion Immune status of the Chinese provincial PNL practitioners appeared fairly good as 86％ of all the workers had received polio-related vaccination,with 41％ of them completed a 3-time inoculation program,when started working in this field.
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Objective To explore the neuroprotective effect of cyclosporine A against cerebral ischemia in a rat model of cerebral ischemia reperfusion. Methods Fifty-two adult male SD rats, weighted 250-280 gram, were randomly divided into three groups: the sham group (group A, n=6), PBS control group (group B, n=23) and cyclosporine A group (group C, n=23). Group C received hypodermic injection of cyclosporine A 10mg/kg daily after surgery and group B re?ceived equal volume of PBS instead. Modified Neurological Severity(mNss)scores were used to assess the neurological deficits at 3, 7, 14, 21 and 30 days following cerebral ischemia. The infarct volume were measured 3 days after reperfu?sion. The neurons, reactive microglia and astrocytes around the infract area were detected by immunofluorescence at 3 and 30 days after surgery. Results Modified Neurological Severity scores were significantly lower in group C than group B at the third(P=0.003),seventh (P=0.011),Fourteenth (P=0.000),twenty-first (P=0.003) and thirtieth (P=0.004) days after surgery. cyclosporine A reduced infarct volume, reactive microglia and astrocytes while increased survived neurons (P<0.001) in ischemic penumbra 3 and 30 days after reperfusion (all P<0.001). Conclusion Continuous injection of cyclosporine A not only protects neurons against ischemia damage but also improves neurological functional recovery af?ter acute stage of damage, possibly through reduction of reactive microglia cells and proliferation of astrocytes.
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To prepare recombinant human retinol binding protein 4 (RBP4) by using the baculovirus expression system and to detect its immunogenicity, the fusion DNA fragment of secretory signal peptide SS64 and human RBP4 gene was subcloned into a baculovirus transfer vector pFastBac-dual(pFBd), and the corresponding recombinant transfer plasmid was transformed into E. coli strain DH10bac, after transposition recombinant shuttle bacmid was screened out. The logarithmic phase Sf9 cells were transfected with the recombinant bacmid and then the recombinant baculovirus containing hRBP4 expression box were generated. After amplification of recombinant baculovirus, the recombinant baculovirus seeds were obtained. To express human RBP4, logarithmic phase Sf9 cells were infected with the virus seeds and SDS-PAGE and Western blotting were used to detect and identify the expression. Finally, to prepare a batch of RBP4 protein, logarithmic phase Sf9 cells in suspension culture were infected with recombinant baculovirus seeds and the supernatant was harvested after 120 hours post-infection for purification. Finally for preparation of polyclonal antibody and evaluation of immunogenicity, the recombinant hRBP4 from insect cells and from E. coli were immunized rabbits. Restriction enzyme digestion and sequencing confirmed that the recombinant baculovirus transfer plasmid was constructed correctly, and subsequently recombinant RBP4-bacmid was generated successfully. SDS-PAGE and Western blotting analysis suggested that human RBP4 protein was highly expressed in Sf9 cells with the molecular weight of approximately 23 kDa. The recombinant RBP4 protein could be secreted into the medium efficiently, and the expression level was calculated amount of 100 mg/L. Finally the rabbit antiserum was harvested after recombinant RBP4 immunization, therein the titer of antiserum against baculovirus recombinant RBP4 is 1:100 000 whereas the titer of antiserum against E. coli recombinant RBP4 is only 1:10 000. Overall, human RBP4 was high efficiently expressed successfully with good antigenicity in baculovirus system, and high affinity antiserum was obtained. A solid foundation was laid for the next step of the preparation of human serum RBP4 detection kit.