Efficacy of relacin combined with sodium hypochlorite against Enterococcus faecalis biofilms

Abstract Objective Relacin is a synthetic molecule that targets RelA, an essential protein in a conserved bacterial stress response system. It was shown to inhibit bacterial growth. The aims of this study were to evaluate the antimicrobial effect of relacin combined with sodium hypochlorite (NaOCl) on Enterococcus faecalis biofilms and to evaluate the cytotoxicity of relacin. Material and Methods 48-h E. faecalis OG1RF biofilms were treated by various concentrations of relacin in order to determine its inhibitory concentration. Then, the 48-h biofilms were treated either with 1-min NaOCl (0.01%, 0.05%) alone, or in combination of relacin. As a means of comparison, the biofilms of ΔrelA were also treated by 1-min NaOCl (0.01%, 0.05%, 0.25%). The treatment efficacy was determined by agar plate count assays. The cytotoxicity of relacin was examined on human gingival epithelial cells Ca9-22 and murine fibroblasts NIH-3T3 by a methyl thiazolyltetrazolium (MTT) assay and a lactate dehydrogenase assay. Statistical analysis was performed by one-way or two-way analysis of variance (ANOVA) with Bonferroni's post-hoc test and an independent Student's t-test. A significance level of p<0.05 was used. Results Relacin inhibited the growth of OG1RF biofilms partially at 8 mM and fully at 14 mM. The relacin (14 mM) and NaOCl combined treatment resulted in significantly higher treatment efficacy than NaOCl treatment alone. At 0.05% NaOCl, the combined treatment resulted in 5.65 (±0.19) log reduction in biofilm viability. The ΔrelA biofilms were more susceptible to NaOCl treatment than the wild type biofilms at 0.25% NaOCl. Relacin at 14 mM was not toxic to host epithelial cells and fibroblasts. Conclusions The combination of relacin with a low concentration of NaOCl was effective and not cytotoxic.

MRI diagnosis of supratentorial solid hemangioblastoma / 实用放射学杂志

Objective To investigate the MRI features of supratentorial solid hemangioblastoma and to improve its diagnostic accuracy.Methods MRI image manifestations of 7 cases with supratentorial solid hemangioblastoma confirmed by operation and pathology were analyzed retrospectively.Results 7 cases of supratentorial solid hemangioblastoma were solitary.All tumors appeared as round or quasi-round masses with clear boundary, and showed equal or long T1 signal and slight long or long T2 signal,the obvious enhancement was seen in all tumors.There was mild to moderate edema around the tumors.The blood vessel was found within or around the tumors.Conclusion MRI manifestations of supratentorial solid hemangioblastoma have certain characteristics.

Clinical research of the level changes of blood lipids,liver function and estrogen in pregnancy patients with intrahepatic cholestasis / 国际检验医学杂志

Objective To explore the predictive values of the levels of blood lipids ,liver function and estrogen for the fetal dis‐tress in intrahepatic cholestasis of pregnancy (ICP) patients .Methods The indicators of blood lipids ,liver function and estrogen of mild IPC group ,severe ICP group and control group were detected respectively ,and the results were analyzed .Results The levels of triglycerides ,total cholesterol and low‐density lipoprotein were positively related to estradiol levels .Multivariate logistic regres‐sion analysis showed that cholyglycine (OR=8 .24 ,P=0 .01) and estradiol(OR=4 .46 ,P=0 .02) were significant for prediction of fetal distress in ICP patients .Conclusion Estradiol and cholyglycine levels may be better indicators for the prediction of fetal dis‐tress in ICP patients .

In vitro study of shaping ability of single-file techniques in curved canals / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To compare the shaping quality in curved canals of two single-file technique systems with other two traditional full-sequential systems.</p><p><b>METHODS</b>Eighty mature molar canals with the curvature between 20 and 45 degrees were randomly divided into four groups. Specimens in each group were prepared to size 25 at working length using A (Reciproc), B (OneShape), C (MTwo) and D (Revo S), respectively. Each canal was scanned by micro-computed tomography before and after preparation. Parameters including changes in dentine volume, percentage of uninstrumented area, degree and tendency of transportation were analyzed. The operating time was also recorded.</p><p><b>RESULTS</b>In full canal length, there was no difference in canal dentine removal, instrumented percentage and transportation degree among four groups (P > 0.05). In the apical 4 mm region, group A removed more dentine [(2.14±0.76) mm(2) of canal surface area and (0.38 ± 0.15) mm(3) of canal volume] than groups B and C(P < 0.05). At 1 mm level, median of transportation degree of group A was 0.05 (0.03)mm, which was smaller than other groups (P < 0.05). Groups A and B took (86.3±24.6) s and (85.9±21.3) s, while groups C and D took (147.4±28.3) s and (126.3±27.7) s srespectively to finish preparation. Single file techniques were significantly faster than the two full-sequential systems (P < 0.01).</p><p><b>CONCLUSIONS</b>Compared with the continuous rotary systems, the reciprocating single-file system A showed better apical shaping ability. Both single-file techniques were more efficient than full-sequential systems for curved canal preparation. Single-file techniques appear to be the effective and efficient method for curved canal preparation.</p>

Effects of electro-acupuncture on behavioral recovery and its molecular basis in chronic spinal cord injured rats / 中国康复理论与实践

@#ObjectiveTo study the therapeutic mechanisms of electro-acupuncture on chronic spinal cord injury in rats.MethodsA model of chronic spinal cord injury was made. After 60 days, the pressure installs were removed, and the animals were treated with electro-acupuncture. A quantitive analysis was carried out using Basso, Beattie, Bresnahan Locomotor Rating Scale (BBB scale). The expression of Neurotrophin-3 (NT-3) and its receptor TrkC were observed through immunohistochemistrical method and western blotting.ResultsAfter spinal cord injury, enhanced NT-3 and TrkC immunoreactivities were evident in neurons and glial cells. Furthermore, they were diminished in the electro-acupuncture group. Meanwhile, both evoked potentials and BBB scales were improved after EA treatment (P<0.05). ConclusionThe treatment of electro-acupuncture may promote behavioral function recovery of experimental animals, and NT-3 and TrkC may take part in the recovery.

MRI Diagnosis of Malignant Breast Tumor / 实用放射学杂志

Objective To evaluate the diagnostic value of malignant breast tumor.Methods 37 patients with malignant breast tumor were scanned by plain scans of MRI and contrast-enhanced MRI.MR findings including shape,border and signal intensity of lesions on plain scans,and the enhanced features after adiministration of Gd-DTPA were analysed and compared with the results of X-ray,B-ultrasound,surgery and pathalogy.Results The correct diagnostic rate of plain scans of MRI and contrast-enhanced MRI was 84.5% and 94.6% respectively.Conclusion MRI is effective medical imaging method in diagnosis of malignant breast tumors.