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OBJECTIVE:To prepare Premna fulva craib liniments and to establish a method for its quality control.METHODS:The liniments was prepared from yellowhairy premna stem,rhizoma drynariae,borneo and menthol in70%al-cohol solvent.Naringin in the liniments was identified qualitatively by TLC,and the content of naringin was determined by HPLC.RESULTS:Naringin was identified in TLC.The sample size of naringin showed a good linear relationship with its peak area in the range of0.51?g～2.55?g(r=0.9993).The average recovery was98.58%(RSD=1.31%).CONCLUSION:The preparation technology is practicable and the method of quality control is reliable.
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Objective To establish a technical process for the separation of octopamine.MethodsTaking the absorptive capacity and elution efficiency of octopamine as indexes,the absorption characteristics and elution parameters of separation with macroporous resin were investigated.Results The static adsorption capacity of H103 type macroporous resin was 2.925mg g-1.The static elution ratio was 98.04%.Conclusion H103 type macroporous resin is effective to separate the octopamine and improve the flavour of fish sauce.
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Objective To establish a HPLC method for the determination of octopamine in fish sauce.Methods A phenomenes luna C18 column was used.The mobile phase was 0.02?g?mL-1 citric acid-0.02?g?mL-1 sodium dihydrogen phosphate(7∶3,v/v) and detection wavelength was 274 nm.Results The linear range of octopamine was 104.0%,RSD=1.53%.The detection limit was 5.7ng?mL-1.Conclusion This method is simple,rapid and reliable.It could be used for the determination of octopamine and its related substances in fish sauce.The content of octopamine in Engraulis japonicus sauce is 1055?g?mL-1.
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AIM: To establish a quality standard for Kudingcha Tablets(Cratoxylum Prunifolium Dyer). METHODS: TLC was adopted for identification of Kudingcha Tablets.RP-HPLC was used to determine the contents of ursolic acid,quercetin and kaempferol in the preparation.The Nova-Pak C_(18) column(3.9 mm?150 mm,4.0 ?m) was used to determine the ursolic acid of content.The mobile phase was methanol-water-H_3PO_4(85∶15∶0.1).The detection wavelength was at 220 nm.The flow rate was 0.8 mL/min and the temperature was at 30 ℃.The Nova-Pak C_(18) column(3.9 mm?300 mm,4.0 ?m) was used to determine the contents of quercetin and kaempferol.The mobile phase was methanol-0.4%H_3PO_4(50:50).The detection wavelength was at 360 nm.The flow rate was 0.7 mL/min.The temperature was at 40 ℃. RESULTS: A good linear relation was obtained when the sample size of ursolic acid was in the range of 0.334-6.672 ?g.The linear range of quercetin was(0.012 99-0.649 6) ?g and the linear range of kaempferol was 0.013 34-0.667 2 ?g.The average recoveries of ursolic acid,quercetin and kaempferol were 97.70%,96.89% and 97.41%,respectively.The RSD were 0.87%,(1.06%) and 1.40%(n=6),respectively. CONCLUSION: This standard is accurate and reliable,and can effectively control the quality of Kudingcha Tablets.