RESUMEN
Objective To study the effects of TGF-? 1 on the proliferation of hepatic stellate cell(HSC) and the interfering effect of serum containing Fufangbiejiaruanganfang (FFBJRGF ) drug in vitro. Methods TGF-? 1 was added to the culture of HSC in vitro and the proliferation of HSC was detected by MTT method. The effects of the serum containing different dosage FFBJRGF on the HSC proliferation was observed by using modified method of serum pharmacology of the traditional Chinese medicine and MTT method. Results The proliferation of HSC was decreased under adding exogenous TGF-? 1, which effect was presented at 72 hours after TGF-? 1 complement. The HSC proliferative reactions were obviously inhibited by adding sera containing high, moderate, low dosage of FFBJRGF as well as IFN-?serum compared with the normal control HSC culture (P
RESUMEN
Objective To investigate the effects of the serum containing Fufangbiejiaruanganfang (FFBJRGF) on cell cycle and apoptosis of hepatic stellate cells(HSC). Methods The influence of TGF-? 1 on cell cycle and rate of apoptosis of HSC in vitro was examined by using flow cytometer, furthermore, the effects of FFBJRGF drug sera on HSC were observed compared with IFN-? serum and normal rat serum controls. Results The numbers in phase of G 0 and G 1 were increased when HSCs were incubated by adding TGF-? 1, IFN-? serum and different dosages of FFBJRGF drug serum respectively, especially being predominant in the FFBJRGP groups. Meanwhile, the intervenient roles in phase of G 2?M?S of HSC were found in FFBJRGF drug serum treatments. Compared with normal serum control group, there were not notable effects on the cellular apoptosis of HSC in the different dosages of FFBJRGF drug serum groups as well as IFN-? group, however, TGF-? 1 could significantly increased HSC apoptosis rate. Conclusion Our results indicates FFBJRGF has anti-hepatic fibrosis efficiency through inhabiting the proliferative cycle of HSC, not up-regulating HSC apoptosis in vitro.