Epidemiological characteristics and changes of D-D and FIB levels in patients with high-altitude pulmonary thromboembolism / 公共卫生与预防医学

Objective The epidemiological characteristics of high-altitude pulmonary thromboembolism (PTE) were investigated, and to enhance people's cognitive level of high-altitude PTE. Methods A tatal of 286 patients with PTE admitted to our hospital from May 2017 to October 2019 were selected and divided into study group (n = 143) living at high altitude of 2500 ~ 4500 m and control group (n=143) living at low altitude of 1500~2450m according to altitude.The clinical data, D-D, FIB levels, and laboratory routine examinations were compared between the two groups, and the risk factors were analyzed using univariate analysis and the independent risk factors were analyzed by multivariate logistic regression. Results The levels of D-D and FIB in the control group were significantly increased (P 0.05). Conclusion  Those were independent risk factors affecting the incidence of PTE at high altitude，including multivariate logistic regression analysis indicated that red blood cell level, HGB level, DVT, D-D, and FIB. The higher the plasma D-D and FIB levels, the recurrence interval, especially the related diseases of patients at high altitude need regular monitoring.

TLR4 activation with LPS inhibits BMP 9-induced osteogenic differentiation of immortalized mouse embryonic fibroblasts / 基础医学与临床

Objective To study the effect of TLR4 activation with LPS on BMP9-induced osteogenic differentiation of immortalized mouse embryonic fibroblasts ( iMEFs).Methods The activation of TLR4/NF-κB signaling path-way was detected by ICC.iMEFs were treated with LPS,BAY11-7082,Adnovirus GFP and BMP9.The early osteo-genic differentiation capability of iMEFs was detected by ALP staining and quantitative assay .The later osteogenic differentiation capability was detected by alizarin red S staining .The expression of later osteogenic differentiation marker gene OCN and OPN were detected by PCR and Western blot .The change of p-Smad1/5/8 was detected by Western blot.The expression of Runx2 and Dlx5 were detected by PCR and Western blot .Results LPS can effec-tively stimulate TLR4/NF-κB signaling pathway .TLR4 activation inhibited BMP 9-induced osteogenic differentiation . BMP9-induced osteogenic differentiation related gene and Smad 1/5/8 signaling activation were inhibited by TLR4 activation .The inhibition effect was partly reversed by BAY 11-7082 ( P<0.05 ) .Conclusions TLR4 activation with LPS can inhibit BMP9-induced osteogenic differentiation of iMEFs cells via NF-κB signaling pathway .