Astragalus polysaccharides improve cardiomyopathy in STZ-induced diabetic mice and heterozygous (SOD2+/-) knockout mice

Oxidative stress plays an important role in the development of diabetic cardiomyopathy. In the present study, we determined whether the effect of astragalus polysaccharides (APS) on diabetic cardiomyopathy was associated with its impact on oxidative stress. Streptozotocin (STZ)-induced diabetic mice and heterozygous superoxide dismutase (SOD2+/-) knockout mice were administered APS. The hemodynamics, cardiac ultrastructure, and the apoptosis, necrosis and proliferation of cardiomyocytes were assessed to evaluate the effect of APS on diabetic and oxidative cardiomyopathy. Furthermore, H2O2 formation, oxidative stress/damage, and SOD activity in cardiomyocytes were evaluated to determine the effects of APS on cardiac oxidative stress. APS therapy improved hemodynamics and myocardial ultrastructure with reduced apoptosis/necrosis, and enhanced proliferation in cardiomyocytes from both STZ-induced diabetic mice and heterozygous SOD2+/- knockout mice. In addition, APS therapy reduced H2O2 formation and oxidative stress/damage, and enhanced SOD activity in both groups of mice. Our findings suggest that APS had benefits in diabetic cardiomyopathy, which may be partly associated with its impact on cardiac oxidative stress.

Study of the malaria situation in forested foothill and nearby plain areas of Myanmar.

A longitudinal demographic-parasitological survey on malaria was conducted at 10 weekly intervals starting from September in one foothill village with the population of 1,095 and one epidemiologically comparable plain village with the population of 962 in Kyauktaga township, Bago division, 120 miles north of Yangon. The objective was to describe and analyse the current malaria situation in a forested foothill area and an adjacent plain area. Ten weekly blood film collections for malaria parasite examination, six monthly sera collections on filter paper for serological examination from the whole study population and ten weekly splenic measurements from 2-9 year children were done. The malaria parasite rate in the foothill area was invariably higher than that in the plain area in all age groups throughout the study period. Moreover, the parasite rate decreased with the increase in distance from the forested foothill area indicating that the deep forest malaria may have some influence on the foothill villages. The total age specific parasite rate in foothill villages was found to be highest in the 5-8 year age group and decreased as the age advanced which may be due to the increasing immunity. The study revealed the presence of local transmission in the foothill village. From these data it is evident that new village sites should be chosen at least 5 miles away from the forest fringe and the malaria control measures in the plain area should utilize chemoprophylaxis and effective chemotherapy focusing on the people who travel into the forest.