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Journal of Experimental Hematology ; (6): 1028-1031, 2007.
Artículo en Chino | WPRIM | ID: wpr-318796

RESUMEN

In order to investigate the expression of heavy chain of HLA-B * 2705 in prokaryotic system and identify its activity, the extra-membrane gene fragment of HLA-B * 2705 was amplified from full-length HLA-B*2705 cDNA by PCR and cloned into pGEM-T vector. After identification by sequencing, the prokaryotic expressing vector pET32a (+)-B * 2705 was constructed. The antigenic activity of expressed protein was identified by Western blot and antibody blocking reaction. The results indicated that the fused HLA-B * 2705 protein expression with high efficiency was obtained. The expressed product was more than 50% of the total bacteria protein. The antigenic activity of expressed protein was confirmed by Western blot and antibody blocking reaction. It is concluded that HLA-B * 2705 fusion protein are obtained as basis for the further studies.


Asunto(s)
Humanos , Escherichia coli , Genética , Metabolismo , Vectores Genéticos , Genética , Antígeno HLA-B27 , Clasificación , Genética , Alergia e Inmunología , Metabolismo , Cadenas Pesadas de Inmunoglobulina , Genética , Alergia e Inmunología , Metabolismo
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