To Detect the Inhibiting Capacity of Bingcha Shuan Contained Serum to HEPG2 Cells' Reproductive Activity (MTT Method) / 浙江中医药大学学报

[Objective] To observe the inhibiting capacity of Bingcha Shuan Containing serum to HEPG2 cells' reproductive activity and to conjecture.Bingcha Shuan has the potential to cure the liver carcinoma.[Method]According to serum pharmacology,HEPG2 cells were cultured in vitro and interfered by various concentrations of Bingcha Shuan contained serum for 24,48 hours respectively.The inhibiting capacity of the Bingcha Shuan contained Serum to HEPG2 cells were detected by MTT.[Result] The Bingcha Shuan contained Serum has the inhibiting capacity to HEPG2 cells in some degree.And there was a marked positive correlation between drug concentration,time and inhibition ratio.[Conclusion] It is suggested that Bingcha Shuan has the inhibiting capacity of cell proliferation and it can provide the support on how to cure the liver cancer of clinic.

Experimental Study of the Effect of Wuyaojing Granules on the Immunological Function of Mice / 医学研究杂志

Objective To observe the effect of Wuyaojing granules(WYJG) on immunological function of mice.Methods Mice were randomly divided into six groups:the high-dosage WYJG group(6.0g/kg),the midst-dosage WYJG group (2.0g/kg),the low-dosage WYJG group(1.0g/kg),the Lindera aggregate group (0.9g/kg),the korean Ginseng group (2.0g/kg) and the normal control group(H2O).After a period of 30-day treatment,the effects of WYJG on the T lymphocyte transformations capacity induced by ConA,delayed hypersensitivity leaded by DNFB,the number of lymphocyte B,serum level of hemolysin and NK activity were observed. Results The high-dosage and the low-dosage WYJG could significantly increase T lymphocyte transformations capacity induced by ConA(P0.05).Conclusion WYJG can significantly increase T lymphocyte transformations capacity induced by ConA and strengthen delay hypersensitivity leaded by DNFB.

Effects of Bingcha Embolus on the proliferation,apoptosis and cell cycle of astrocytes / 中成药

AIM: To study the effects of Bingcha Embolus on the proliferation, apoptosis and cell cycle of astrocytes in vitro in terms of serum pharmacology. METHODS: The cerebral cortex astrocytes of neonatal Sprague-Dawley rats were cultivated in vitro, and the purified astrocytes were randomly devided into Bingcha Embolus groups (high dose, middle dose, low dose) and control group after being identified by means of immunocytochemical method. After the incubation of 48 h with sera, MTT was employed as a proper method to detect the proliferation of astrocytes. The ratio of apoptosis and different cell cycle phases were measured by Flow Cytometry. RESULTS: The results of MTT showed that the cell number of Bingcha Embolus groups (high dose, middle dose) were obviously fewer than that of the control group (P