RESUMEN
Objective: To analyze the change of chemical constituents of Polygoni Multifori Radix from different harvest time. Methods: Fifteen batches of Polygoni Multifori Radix samples from different harvest time were determined by UPLC-Triple TOF-MS/MS. Through the analysis of the multistage tandem mass spectrometry, the characteristic peaks were extracted with mass spectrometry data peak matching, peak alignment, and noise filtering. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) were used for data processing. The components were identified according to a mass spectrometry accurate mass and two mass spectrometry fragmentation information, combined with the software of database search and literature. Results: The chemical composition in Polygoni Multifori Radix samples from different harvest time are clearly distinguished. Twenty-four kinds of differential chemical constituents were identified. Among them, there are nine kinds of common differential constituents which presented different changing laws. Conclusion: This study provides the basic data for revealing the dynamic change law of metabolite accumulation of Polygoni Multifori Radix and confirms its optimal harvest period.
RESUMEN
This work was designed to investigate proteins differentially expressed in cultivated Pseudostellaria heterophylla and its wild type using iTRAQ proteomics approach. The extracted proteins were digested using FASP method and identified by iTRAQ coupled with LC-MS/MS technology and then analyzed by Protein Pilot 5.0 search engine. Proteins differentially expressed were searched through comparison of relatively quantified proteins. The analysis was conducted using GO (gene ontology), KEGG and STRING. A total of 3 775 proteins were detected, among them, 3 676 proteins can be quantified, of which 127 proteins were up-regulated and 205 were down-regulated in cultivated Pseudostellaria heterophylla. We found 71 significantly differentially expressed proteins for further analysis. These proteins were classified into nine categories:heat shock proteins, transferases, oxidoreductases, lyases, isomerases, ligases, hydrolases, tubulin and translocases. The results indicated that the carbohydrate and cellular amino acids metabolism of cultivated Pseudostellaria heterophylla were weaker than its wild type and its ability of responding to stress was much stronger. GWD1, PHS1, GBE1, PGM, and BAM1 are the important proteins to regulate sucrose; metE and CYS are the key proteins that regulate amino acids in cultivated and wild Pseudostellaria heterophylla. This will provide the basic information for exploring the cause of secondary metabolites differences in different ecotype of Pseudostellaria heterophylla and the protein mechanism of its quality formation.
RESUMEN
Objective: To study the effect of different processing methods and conditions for bioactive constituents in Polygoni Multiflori Radix (the root tubers of Polygonum multiflorum) and their change regularity, in order to optimize and establish appropriate drying methods and conditions. Methods: The stilbene glucoside, free-anthraxquinones, combined-anthraxquinones, and catechin from 12 kinds of Polygoni Multiflori Radix in different processing methods were determined by MEKC-DAD. The multiple functional substances of principal component analysis (PCA) was made to standardized treatment for the comprehensive evaluation of the 12 samples by different drying methods. Results: The contents of multiple functional substances in different processed products from Polygoni Multiflori Radix presented the certain regularity. The contents of stilbene glucoside and combined anthraquinone were the highest in Polygoni Multiflori Radix which was cut into thick pieces dried in the sun, the total free anthraquinones were the highest in Polygoni Multiflori Radix which was cut into thick pieces dried at 70℃, the content of catechin was the highest in Polygoni Multiflori Radix which was cut into thin pieces dried at 40℃. The comprehensive evaluation index obtained with PCA showed that the sample cut into thick pieces dried in the sun was significantly higher than those with other samples. Conclusion: The influence of processing methods by the determination of multiple functional substances in Polygoni Multiflori Radix has been investigated and a scientific basis for the suitable processing method of Polygoni Multiflori Radix provided.
RESUMEN
A comprehensive analytical method based on UPLC-MS/MS was developed for the simultaneous determination of thirteen components including three stilbenes (stilbeneglucoside, polydatin, resveratrol), four anthraquinones (emodin, physcion, emodin-8-β-D-glucopyranoside, aloe-emodin), five flavonoids (epicatechin, rutin, hyperoside, astragalin,quercetin) and one phenolic acid (gallic acid) in Polygoni Multifori Caulis.The separation was carried out on a Waters BEH C18 column（2.1 mm×100 mm,1.7 μm）with gradient elution of acetonitrile-water (0.1% acetic acid) at a flow rate of 0.25 mL•min⁻¹, and column temperature was 35 ℃. The target compounds were analyzed by multiple reaction monitoring (MRM) mode. TOPSIS analysis ware performed to evaluate the samples from different areas and commercial herbs according to the contents of thirteen components. The correlation coefficients of all the calibration curves were higher than 0.991 5. The average recoveries ranged from 95.24% to 102.3%, and the relative standard deviations were less than 5%. The result of TOPSIS analysis showed that the comprehensive quality of Polygoni Multifori Caulis sample from Guangzhou was better. The developed method with good repeatability and accuracy was suitable for the simultaneous determination of multiple functional substances, which provided a new basis for the comprehensive assessment and overall control of the quality of Polygoni Multifori Caulis.
RESUMEN
Objective: To establish a method for simultaneous analysis on the inorganic elements in Polygoni Multiflori Radix. by inductively coupled plasma mass spectrometry (ICP-MS) and compare the inorganic elements in Polygoni Multiflori Radix between different origin and commercial herbs. Methods: The sample solutions were analyzed by ICP-MS after microwave digestion. The data of correlations, principal components, and cluster were analyzed with the SPSS 16.0 software. Results: Twenty four inorganic elements in Polygoni Multiflori Radix were determined. And there were some correlations among the inorganic elements in Polygoni Multiflori Radix. The contents of K, Fe, Mg, Ca, and P were abundant. The content of heavy metals and harmful elements should be caused for concern. Six main factors were selected by principal component analysis (PCA). The PCA results showed that Fe, Si, Ca, Al, K, Be, Sb, Mn, Zn, and Ba may be the characteristic elements in Polygoni Multiflori Radix. Conclusion: This experiment provides the evidence for the quality control and safety evaluation of Polygoni Multiflori Radix.
RESUMEN
OBJECTIVE: To establish a micellar electrokinetic chromatography with diode array detection (MEKC-DAD) method for simultaneous determination of seven components including stilbene glucoside, emodin, aloe-emodin, rhein, chrysophanol, physcion, and catechin in Polygoni Multiflori Radix, of different origins and commercial herbs. METHODS: Based on the mode of MEKC, an uncoated fused silica capillary (75 μm × 64.5 cm, 56 cm of effective length) was adopted, and 25 mmol · L-1 borax-30 mmol · L-1 SDS-10% acetonitrile (pH 9.60) was selected for the running buffer. The detection wavelength was set at 254 nm. The separation voltage was 25 kV and the column temperature was maintained at 24℃. The sample was injected at 5 kPa for 5 s. RESULTS: The calibration curves of the seven components in Polygoni Multiflori Radix showed good linearity (r ≥ 0.9994). The average recoveries of the method were between 99.164%-101.504%. CONCLUSION: The established method is reliable and accurate, and can be used for the quality control of Polygoni Multiflori Radix.
RESUMEN
To study the dynamic change law of bioactive constituents from Polygonum multiflorum, and to explore the optimal harvest period of P. multiflorum. Determination of stilhene glucoside, anthraquinones and catechin from P. multiflorum in different harvest times by MEKC-DAD, and principal component analysis (PCA) was used to comprehensive evaluation for bioactive constituents. There are obvious differences among the contents of active ingredients in various collecting periods samples, the content of stilbene glucoside was the highest in November, the total content of combined anthraquinone was the highest in November and December, the content of catechin was the highest in September. The comprehensive evaluation index obtained with principal component analysis showed that the sample collected in November is significantly higher than those with other samples. The optimal harvest period of P. multiflorum is November.
Asunto(s)
Electroforesis , Fallopia multiflora , Química , Metabolismo , Factores de TiempoRESUMEN
OBJECTIVE: To explore the dynamic changes of the inorganic elements in Polygoni Multiflori Radix.