Perception and attitude of the healthcare workers on workplace violence in a secondary Grade A hospital: based on the perspectives of a qualitative interview / 中国职业医学

OBJECTIVE: To understand the true perception and attitude of the healthcare workers(HCWs) on the workplace violence(WPV) in a secondary Grade A hospital.METHODS: A total of 27 HCWs in a secondary Grade A hospital were selected as study subjects using a typical sampling method. Semi-structured interviews were conducted to collect the information of their perception and attitude on the types and effects of WPV, as well as on the coping measures and the work stress. The characteristics of WPV in the hospital were analyzed. RESULTS: The interviewees believed that verbal abuse was the most common type of WPV received by HCWs. Nurses and female workers were the main victims of WPV. Horizontal violence among colleagues and sexual harassment should not be ignored. Common causes of violent incidents for patients and their family members against HCWs included the fees for treatment, doctor-patient communication, patients alcohol abuse and treatment outcomes not meeting patients′ expectation. About half of the interviewees said that WPV had serious impact on their mental health. The follow-up coping measures to violent incidents were mostly to reassurance to patients with unconditional apology from the HCWs. More than half of the interviewees considered that they had acceptable workload, while some interviewees considered their work to be stressful. The interviewees suggested that improving doctor-patient communication, hospital systems, and the professional knowledge of HCWs were the recommended means to prevents and control the WPV. CONCLUSION: The perception and attitude of HCWs on WPV in this hospital are relatively scattered. Considering the complex causes, the serious consequences, and difficult coping measures, the hospitals, HCWs and all sectors of society need to take comprehensive measures to prevent WPV towards HCWs.

Status and influencing factors of psychological violence against healthcare workers in a secondary Grade A hospital / 中国职业医学

OBJECTIVE: To study the status and influencing factors of psychological violence in healthcare workers(HCWs) of a secondary Grade A hospital. METHODS: A total of 1 028 HCWs in a secondary Grade A hospital were selected as the study subjects in 2019 using a typical sampling method. The psychological violence they had received in the previous year was investigated using the Workplace Violence in the Health Sector Country Case Studies Research Instruments--Survey Questionnaire. The results were compared with the baseline survey results in 2018. RESULTS: The incidence of psychological violence in the workplace of HCWs in 2019 was 41.6%, among which the incidence of verbal abuse, bullying/gang bullying, sexual harassment and racial discrimination were 40.2%, 9.9%, 1.9% and 1.6%, respectively. The incidence of verbal abuse, bullying/gang bullying and psychological violence among HCWs in 2019 were decreased compared with that in 2018(45.7% vs 40.2%, 12.8% vs 9.9%, 47.1% vs 41.6%, all P<0.05). The binary logistic regression analysis results showed that the HCWs in medical technology, internal medicine, surgery, outpatient and emergency departments were at higher risk of verbal abuse than that in administrative departments(all P<0.01). The HCWs in direct contact with patients had a higher risk of verbal abuse and psychological violence(all P<0.01). The HCWs aged <35 and those with night-shift-work had higher risks of bullying/gang bullying(all P<0.05). Male HCWs had a higher risk of sexual harassment than female HCWs(P<0.05). The coping style of attempting to conceal the occurrence of the incident after HCWs suffered psychological violence dropped from 5.8% in 2018 to 0.5% in 2019(P<0.01), and the proportion of no response was as high as 59.1%. CONCLUSION: The psychological violence of HCWs in this hospital is very serious and has specific features. The incidence of psychological violence decreased compared with that a year ago, but there is still room for improvement. The influencing factors of psychological violence are complex and the consequences are serious. Therefore, it is urgent for HCWs and hospital management departments to improve their management methods.

Expressions of CSE and CBS in the penile corpus cavernosum of hyperglycemia rats and their implications / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the impact of hyperglycemia on the hydrogen sulfide (H2S) signaling pathway in rat penile tissue and its relationship with erectile function.</p><p><b>METHODS</b>Twenty healthy male Sprague Dawley (SD) rats aged 8 weeks were randomly divided into groups A (4-week healthy control), B (4-week diabetes mellitus model), C (6-week healthy control) and D (6-week diabetes mellitus model). The rats in groups B and D were injected intraperitoneally with streptozotocin at 50 mg/kg to induce diabetes mellitus, while those in groups A and C with the same volume of normal saline. The animals were killed at 4 (groups A and B) and 6 weeks (groups C and D) after treatment for measurement of the maximal intracavernous pressure/mean arterial blood pressure (ICP(max)/MAP) by electrostimulation, determination of the H2S concentration in the plasma and penile tissue, and detection of the expressions of cystathionine-beta-synthetase (CBS) and cystathionine-gamma-lyase (CSE) in the penile corpus cavernosum by immunohisto- chemistry and Western blot.</p><p><b>RESULTS</b>With electrostimulation of the pelvic ganglia at 5V and 7 V, ICP(max)/MAP was significantly reduced in groups B (0.19 +/- 0.03 and 0.29 +/- 0.04) and D (0.14 +/- 0.04 and 0.25 +/- 0.04) as compared with A (0.46 +/- 0.07 and 0.68 +/- 0.09) and C (0.43 +/- 0.07 and 0.65 +/- 0.16) (P < 0.05). No statistically significant differences were found in the level of serum testosterone either between groups A and B ([469.19 +/- 126.46] ng/dl vs [359.08 +/- 60.06] ng/dl, P > 0.05) or between C and D ([470.44 +/- 209.28] ng/dl vs [297.01 +/- 96.58] ng/dl, P > 0.05). Groups B and D showed remarkable reduction in the H2S concentration (P < 0.05) and the expressions of CBS and CSE (P < 0.05) in comparison with A and C, and the CBS and CSE expressions were even more significantly decreased in D than in B (P < 0.05).</p><p><b>CONCLUSION</b>The reduced concentration of H2S and decreased expressions of CBS and CSE in the penile corpus cavernosum of the diabetic rats suggested that the H2S signaling pathway might be involved in hyperglycemia-induced erectile dysfunction.</p>

Endogenous hydrogen sulfide and androgen deficiency-induced erectile dysfunction in rats / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the role of endogenous hydrogen sulfide (H2S) in erectile dysfunction (ED) induced by androgen deficiency.</p><p><b>METHODS</b>We randomly divided 30 eight-week-old healthy male SD rats into six groups: 2-week control (A), 4-week control (B), 2-week castration (C), 4-week castration (D), 2-week castration + androgen replacement (E), and 4-week castration + androgen replacement (F), those in groups E and F subcutaneously injected with testosterone propionate (TP) at the physiological dose of 3 mg/kg per day after castration, while those in the other groups with isodose oil instead. At 2 and 4 weeks after operation, we determined the level of serum testosterone (T) , intracavernous pressure (ICP) , mean carotid arterial pressure (MAP) of the rats, measured the concentration of H2S in the plasma and corpus cavernosum tissue, and detected the expressions of cystathionine-P3-synthase (CBS) and cystathionine-gamma-lyase (CSE) by immunohistochemistry and Western blot.</p><p><b>RESULTS</b>The serum T level was significantly lower in group C ([0.63 +/- 0.15] nmol/L) than in A ( [ 16.55 +/- 4.17] nmol/L) and E ( [ 18.99 +/- 4.62] nmol/L) (P <0.05), as well as in group D ([0.70 +/-0.22] nmol/L) than in B ([15.44 +/-5.18] nmol/L) and F ([20.99 +/-6.41] nmol/L) (P <0. 05) , and so were ICP/MAP after 5 and 7 V electrical stimulation of the pelvic ganglia (P <0. 05) , H2 S concentration (P <0.05), and the expressions of CBS and CSE (P <0.05). The expressions of CBS and CSE proteins were also significantly decreased in group C as compared with D (P <0.05).</p><p><b>CONCLUSION</b>The reduced expressions of CBS and CSE may inhibit the H2 S signaling pathway, which might be one of the mechanisms underlying androgen deficiency-induced ED in rats.</p>

Hydrogen sulfide and penile erection / 中华男科学杂志

Hydrogen sulfide (H2S) is the third type of active endogenous gaseous signal molecule following nitric oxide (NO) and carbon monoxide (CO). In mammalians, H2S is mainly synthesized by two proteases, cystathionine-beta-synthase (CBS) and cystathionine-gamma-lyase (CSE). H2S plays an essential function of physiological regulation in vivo, and promotes penile erection by acting on the ATP-sensitive potassium channels to relax the vascular smooth muscle as well as by the synergistic effect with testosterone and NO to relax the corpus cavernosum smooth muscle (CCSM). At present, the selective phosphodiesterase type 5 (PDE5) inhibitor is mainly used for the treatment of erectile dysfunction (ED), but some ED patients fail to respond. Therefore, further studies on the mechanism of H2S regulating penile erection may provide a new way for the management of erectile dysfunction.

Main sources of dietary calcium in perimenopausal women in Changsha / 中南大学学报(医学版)

OBJECTIVE@#To describe and analyze the main food sources of dietary calcium intake in perimenopausal women in Changsha,China.@*METHODS@#Twenty-four hour recall method was used for 3 consecutive days to collect food intake information. Total calcium intake and proportion of each food were calculated.@*RESULTS@#The average calcium intake was (506.61+/-33.94)mg,and calcium intake in only 6.25% women reach standard. The main sources of dietary calcium were vegetable, milk, wheat, meat, and fruits. Thirty-three kinds of food, including 15 vegetables, 3 bean and its products, 4 kinds of milk, 5 kinds of wheat, 3 kinds of eggs, 1 kind of oil and 1 kind of fruit supplied about 90% of the dietary calcium.@*CONCLUSION@#The average calcium intake is low in perimenopausal women in Changsha. To improve the situation, we should increase intake of milk, bean, and its products, with calcium supplementation among these people.

Effect of resveratrol on the cognitive ability of Alzheimeros mice / 中南大学学报(医学版)

OBJECTIVE@#To explore the effect of resveratrol on the cognition of Alzheimer's mice (AD) and its mechanism, and to assess its action on the reproduction system.@*METHODS@#According to the results of step-down test, 84 Kunming female mice were randomly divided into 6 groups: Group A [sham operated+1% CMC-Na (0.01 mL/g)], Group B [ovariectomy+D-galactose+1% CMC-Na (0.01 mL/g)], Group C [ovariectomy+D-galactose+0.05 mg/(kg.d) Diethylstilbestrol], Group D [ovariectomy+D-galactose+15 mg/(kg.d) Res], and Group E [ovariectomy+D-galactose injected+45 mg/(kg.d) Res], and Group F [ovariectomy+D-galactose +135 mg/(kg.d) Res]. Experimental cycle was 60 days.@*RESULTS@#Resveratrol of every dosage could improve the performance records of behavior tests in AD mice,could inhibit the SOD vitality and the MDA level both in the serum and in the brain, and could suppress the acetylcholinesterase vitality and the bax expression. Resveratrol has no endometrial hyperplasia effect.@*CONCLUSION@#Resveratrol can improve the cognitive ability of AD mice, which may contribute to the resveratrol's antioxidation and antiapoptosis, and can modulate acetylcholinesterase. Resveratrol has no side-effect of endometrial hyperplasia on AD mice.