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Background and purpose:Colorectal cancer(CRC)is one of the common malignancies,but the mechanism by which it develops resistance to drug remains unclear.The tumor microenvironment(TME),especially cancer-associated fibroblast(CAF),plays an important role in the occurrence,development and drug resistance of tumors.This study aimed to investigate the effect of inflammatory cancer-associated fibroblasts(iCAF)on drug resistance in CRC cells and its possible mechanism.Methods:The primary CAFs were collected from CRC patients underwent surgery in Putuo Hospital,Shanghai University of Traditional Chinese Medicine from Aug.2022 to Sep.2022,and the primary cells were sorted according to the surface marker of CAF[approved by the Ethics Committee of Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine:PTEC-A-2023-5(S)-1],platelet derived growth factor receptor alpha(PDGFRA),to screen iCAF.Human intestinal fibroblast(HIF)and iCAF cells were cultured using serum-free medium to obtain conditioned medium.According to the treatment method,colon cancer cells were divided into control group(no treatment),experimental group 1(treated with HIF-CM)and experimental group 2(treated with iCAF-CM).We observed the changes in the survival rate and apoptotic rate of CRC cells,the changes in protein and mRNA levels and the effect on the Wnt/β-catenin signaling pathway after stimulation with HIF-CM or iCAF-CM.Results:After iCAFs stimulation,the half inhibition concentration(IC50)of CRC cells was higher compared with the control group and HIF-CM group(P＜0.05).Compared with the control group and HIF-CM group,the apoptotic rate of tumor cells in iCAF-CM group decreased significantly,the expression of caspase-3 was decreased,and the expressions of Bcl-2,Bcl-xL and survivin were increased(P＜0.05).The Wnt/β-catenin signaling pathway was activated in the iCAF-CM group.Conclusion:iCAFs can mediate drug resistance in CRC cells,and the mechanism is related to the activation of Wnt/β-catenin signaling pathway.
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Objective To evaluate the schistosomiasis control effect of the comprehensive measures that focused on beach?land smoothing and tillage cultivation in Tieban Marshland,Wuhan Section of the Yangtze River. Methods From 2011 to 2015, before and after the comprehensive control,the Oncomelania hupensis snail situations in Tieban Marshland were investigated in spring annually,and the residents aged 6-65 years in Huayuan Community which was next to the marshland were examined to un?derstand their schistosome infection status. In late July,2014,the sentinel mouse surveillance was carried out. Moreover,the eco?nomic effectiveness of comprehensive development was evaluated. Results The schistosome infection rates of residents in 2011 (before the comprehensive control),2012(after the comprehensive control),and 2013 were 0.72%(3/414),0.37%(2/536)and 0.31%(1/326),respectively,and no schistosomiasis patients were found in 2014 and 2015. The snail area,the occurrence rate of frames with snails,and the average density of living snails in Tieban Marshland in 2005 decreased by 22.18%,97.83% and 98.25%,respectively compared to those in 2011,and no schistosome infected snails were found. No positive mice were found in the sentinel mouse surveillance. The annual net income of Tieban Marshland increased by 233.33%compared to that before the comprehensive development. Conclusion The endemic situation of schistosomiasis and snail situation declined significantly af?ter the comprehensive development in Tieban Marshland,and the economic and social benefits are significant.
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OBJECTIVE@#To study a surgical and repairing method for defects after the resection of basal cell carcinoma of external nose.@*METHOD@#There are 26 cases of basal cell carcinoma that tumors have been resected completely after operation. For defect repairing in those 26 cases, 2 cases adopt direct suture method; 2 cases use skin graft repairing methods; 18 cases employ naselabial skin flap repairing method; 4 cases choose forehead pedicle skin flap repairing methods.@*RESULT@#the wound of all the 26 cases was primary healed. Additionally, skin flap and skin graft were all survived. Follow-up studied in patients 1 to 3 years after the surgery showed that the local scar was not obvious and no tumor recurred or transferred.@*CONCLUSION@#Different surgical and repairing methods are performed to obtain a satisfactory results based on the area of defect and its location in nose. Naselabial skin flap is especially an ideal method to repair defects.