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1.
Braz. j. med. biol. res ; 56: e12938, 2023. tab, graf
Artículo en Inglés | LILACS-Express | LILACS | ID: biblio-1447687

RESUMEN

Brucellosis has become a global zoonotic disease, seriously endangering the health of people all over the world. Vaccination is an effective strategy for protection against Brucella infection in livestock in developed countries. However, current vaccines are pathogenic to humans and pregnant animals, which limits their use. Therefore, it is very important to improve the safety and immune protection of Brucella vaccine. In this study, different bioinformatics approaches were carried out to predict the physicochemical properties, T/B epitope, and tertiary structure of Omp2b and Omp31. Then, these two proteins were sequentially linked, and the Cytotoxic T lymphocyte associated antigen-4 (CTLA-4) variable region was fused to the N-terminal of the epitope sequence. In addition, molecular docking was performed to show that the structure of the fusion protein vaccine had strong affinity with B7 (B7-1, B7-2). This study showed that the designed vaccine containing CTLA-4 had high potency against Brucella, which could provide a reference for the future development of efficient brucellosis vaccines.

2.
Arq. bras. med. vet. zootec. (Online) ; 71(1): 1-10, jan.-fev. 2019. tab, graf
Artículo en Inglés | LILACS, VETINDEX | ID: biblio-989374

RESUMEN

In this study, neem leaves were successively extracted with petroleum ether, 95% ethanol and water and the insecticidal activities of these extracts against Oxya chinensis larvae were measured. The results showed that 95% ethanol extract gave the highest extraction yield and insecticidal activity, and it was further extracted with five different solvents. The petroleum ether extract from the 95% ethanol extract possessed the highest insecticidal activity with median lethal concentration values ranging from 14.93 to 55.66mg/mL. The gas chromatography-mass spectrometer analysis showed that the petroleum ether extract mainly composed of alkanes, olefin, esters and amide. The pathological examination revealed that the prominent lesions, including reduced regenerative cells in midgut and swelled and degenerated cylindrical cells, were observed in the 5th instar Oxya chinensis after treatment. The ultrastructural features showed that the cylindrical cells, microvilli and mitochondria were seriously damaged. These results suggested that the petroleum ether extract from neem leaves had potent insecticidal activity and could be a candidate insecticide.(AU)


Nesse estudo, folhas "neem" foram extraídas com sucesso com éter de petróleo, 95% de etanol e água, e as atividades inseticidas desses extratos foram medidas contra larvas de Oxya chinesis. Os resultados mostram que extrato com 95% de etanol deram o maior resultado de extração e atividade inseticida e foi então extraído utilizando mais cinco diferentes solventes. O éter de petróleo do extrato de 95% etanol apresentou maior atividade inseticida com concentração letal média variando de 14.93 a 55.66mg/mL. A análise por cromatografia de massa mostrou que o extrato de éter de petróleo está composto principalmente de alcanos, alcenos, ésteres e amidas. A avaliação patológica revelou que as lesões proeminentes, inclusive células regenerativas reduzidas no intestino e células cilíndricas edemaciadas e degeneradas foram observadas no quinto estágio de desenvolvimento da Oxya chinesis após tratamento. As características ultraestruturais mostraram que as células cilíndricas, microvilos e mitocôndrias apresentavam lesões graves. Esses resultados sugerem que o extrato de éter de petróleo de folhas de "neem" tem atividade inseticida potente e pode ser um candidato a inseticida.(AU)


Asunto(s)
Azadirachta/fisiología , Insecticidas/análisis
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