Establishment and optimization of the regeneration system for common dandelion (Taraxacum officinale Weber) / 生物工程学报

A protocol is presented for direct and indirect regeneration of common dandelion (Taraxacum officinale Weber) from leaf and petiole explants. Multiple shoots were obtained on MS medium containing 0.2 mg/L IAA and 1 mg/L TDZ. For indirect regeneration, fragile calli were obtained from leaf and petiole explants on MS medium supplemented with 0.5 mg/L 2,4-D and 2.0 mg/L 6-BA. Regenerated plantlets were obtained when these calli were cultured on MS medium containing 1.0 mg/L 6-BA. Random amplified polymorphic DNA (RAPD) analysis of nine regenerated plantlets revealed 61 scorable bands from 10 primers, including three specific bands.

Establishment and optimization of in vitro regeneration system for Plantago major L / 生物工程学报

Plantago major is not only used as medicinal herb but also an important model plant of ecology. Little work has been reported on the tissue culture of P. major. A reproducible system for direct shoot morphogenesis and callus induction of Plantago major L. 'Giant Turkish' was described. Using seed as explants, the adventitious buds were obtained 4 to 5 weeks following incubation on MS medium supplemented with 0.2 mg/L IAA and 1.0 mg/L TDZ. The frequency of adventitious buds was as high as 100%. The average number of buds per explant was 14.6. Random amplified polymorphic DNA analysis on 9 regenerants indicated that somaclonal variation occurred at DNA level. Using leaves as explants, calli were easily induced on MS medium supplemented with 1.0 mg/L NAA 3 weeks following inoculation. The frequency of callus induction can be as high as 98%. On MS medium containing 4.0 mg/L 6-BA, 25% of calli differentiated and the mean number of buds per piece of callus was 2.8. The buds developed roots on 11/2 MS medium and formed plantlets, 90% of which survived when transplanted to greenhouse.