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Objective To discuss the effect of 5-Aza-CdR on pancreatic carcinoma Capan-2 cell proliferation and PCDH8 gene expression.Methods Pancreatic carcinoma Capan-2 cells were treated with different doses of 5-Aza-CdR with or without gemcitabine,negative control group without drug,0.08μmol/L group with 0.08μmol/L 5-Aza-CdR,0.40 μmol/L group with 0.40 μmol/L 5-Aza-CdR,2.00 μmol/L group with 2.00 μmol/L 5-Aza-CdR,10 μmol/L group with 10 μmol/L 5-Aza-CdR,50 μmol/L group with 50 μmol/L 5-Aza-CdR. The inhibition ratio of Capan-2 cell proliferation were observed by MTT assay and PCDH8 gene and protein expression were detected by RT-PCR and Western blot. Results The inhibition ratio was increased with 5-Aza-CdR dose increasing(P<0.01),but decreased apparently with times extending(P<0.01). Inhibition ratio in 5-Aza-CdR and gemcitabine group was higher than those with only 5-Aza-CdR or gemcitabine groups(P<0.05 or P<0.01).The levels of PCDH8 gene and protein expression were increased significantly in 5-Aza-CdR treatment groups,with dose dependent (P <0.01 ).Conclusion 5-Aza-CdR can inhibit the proliferation of pancreatic carcinoma Capan-2 cell proliferation,and increase PCDH8 gene expression. The inhibition effect is strong when combined with gemcitabine.
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Objective To study the impact of maternal and infant bedside nursing on promoting the parenting skills of puerperae and their families. Methods A total of 421 pairs of mothers and infants hospitalized in our department from June to July 2013 were selected as the observation group and received maternal and infant bedside nursing; all the nursing procedures and health education were conducted at the bedside for the mothers and neonates by responsible nurses and relevant publicity materials were given out. 410 pairs of mothers and infants hospitalized in our department from June to July 2011 were selected as the control group and received traditional nursing. Results The satisfaction de-gree of the observation of was 96.43% and that of the control group was 89.76%, with statistically significant difference (P<0.01); the puerperae and their families of the observation group had significantly better parenting skills than the control group. Conclusion Maternal and infant bedside nursing can increase the nursing satisfaction and promote the parenting skills of puerperae and their families, thereby worthy of clinical promotion and application.