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Journal of Southern Medical University ; (12): 466-468, 2006.
Artículo en Chino | WPRIM | ID: wpr-255283

RESUMEN

Primer Express 2.0 software was used to design the primers and the MGB probe. With the plasmid pHaMDR1/A containing mdr1 cDNA as the template, we established a real-time fluorescent quantitative polymerase chain reaction system, which, at the template concentration of 3.061 x 10(3) to 3.061 x 10(9) cps/ml, had a correlation coefficient of 0.988243 between template concentration and threshold cycle value. This PCR method allows sensitive, specific and quantitative detection of human mdr1 gene.


Asunto(s)
Femenino , Humanos , Masculino , Miembro 1 de la Subfamilia B de Casetes de Unión a ATP , Genética , Cartilla de ADN , Colorantes Fluorescentes , Fluorometría , Métodos , Genes MDR , Genética , Reacción en Cadena de la Polimerasa , Métodos , Polimerasa Taq
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