Chemical Constituents of Ganoderma resinaceum / 中草药·英文版

Objective To study the chemical constituents of Ganoderma resinaceum. Methods Chemical constituents were isolated and purified by silica gel column, Sephadex LH-20, and ODS column chromatography. The structures were identified by means of physicochemical and spectral data. Results From the CHCl3 part of the fruiting bodies of this material, eight compounds were isolated. Their structures were determined to be ergosterol (1), β-sitosterol (2), ganoderic acid E (3), ganoderic acid G (4), ganoderic acid C2 (5), ganoderic acid B (6), ganoderic acid A (7), and lucidenic acid A (8). Conclusion All the compounds were obtained from this fungus for the first time.

Chromatographic fingerprints of triterpenoid constituents of Ganoderma lucidum / 中国中药杂志

<p><b>OBJECTIVE</b>To establish chromatographic fingerprints of triterpenoid compounds in ganoderma RP-HPLC for the quality control of ganoderma.</p><p><b>METHOD</b>HPLC fingerprints were established. The chromatographic conditions were as follows: the separation was performed on a Diamonsil C18 column (4.6 mm x 250 mm, 5 microm), using a gradient elution with acetonitrile-water containing 0.8% perchloric acid as the mobile phases. The flow rate was 0.9 mL x min(-1), the detection wavelength was 254 nm and the temperature of column was at ambient temperature. The common patterns of HPTLC and HPLC fingerprints were separately obtained through "Chromafinger" solution software, and quality assessments were analyzed by similarity.</p><p><b>RESULT</b>The HPLC fingerprint pattern of G. lucidum consists of 18 peaks, among them 6 peaks were identified by chemical reference substances.</p><p><b>CONCLUSION</b>This study provided theory and experimental data in order to comprehensively evaluate the quality of ganoderma.</p>

Content Determination of Monotropein in the Different Parts of Morinda officinalis and Its Counterfeit Species / 中药新药与临床药理

Objective To determine the content of monotropein in the different parts of Morinda officinalis and in its counterfeit species.Methods The method of HPLC was used with chromatographic conditions as follows:Kromasil C18(150 mm?4.6 mm,5 ?m) column,the mobile phase of methol-0.4 %phosphate solution(5:95→28.8:71.2,15 min),the velocity of flow being 1 mL/min,the detection wavelength at 231 nm and column temperature being 25 ℃.Results The content of monotropein in the leaves and radix of Morinda officinalis and Morinda parvifolis is the highest.Conclusion It is reasonable to replace the root of Morinda officinalis with the leaves of Morinda officinalis and Morinda parvifolis for the monotropein extraction.