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Objective To investigate the clinical significance of CUL4B and interleukin?6( IL?6) levels in patients with multiple myeloma(MM).Methods The mRNA of CUL4B and IL?6 were detected by real?time relative quantitative PCR and enzyme?linked immunosorbent assay( ELISA) in 46 cases of MM and 40 cases of non hematologic cancer patients.Results CUL4B concentrations in patients with MM were 3.455(2.098,4. 768).IL?6 concentrations in patients with MM were significantly higher than those of the normal control group ((23. 985(23. 015,26. 878) ng/L vs. 6. 205(5. 405,10. 215) ng/L,Z=-8. 262,P=0. 000). The expression of CUL48 mRNA in Stage Ⅰ,Ⅱ,Ⅲ was 2. 424 ( 1. 881, 3. 583 ) , 3. 594 ( 2. 093, 5. 738 ) , 4. 300 ( 2. 928, 7. 272) respectively, and IL?6 was 23. 115 ( 22. 723, 23. 568 ) , 23. 630 ( 22. 860, 26. 625 ) , 26. 35 ( 24. 995, 30. 550) ng/L respectively,with the increase of clinical stage,the level of CUL4B and IL?6 showed increasing trend( P<0. 05) . CUL4B concentrations were significantly decreased in MM patients after treatment than before treatment(1. 665(1. 420,2. 298) vs. 3. 455(2. 098,4. 768),Z=-4. 955,P=0. 000). IL?6 concentrations were significantly decreased in MM patients after treatment than before treatment( 15. 160( 11. 705,17. 195) ng/L vs. 23. 985(23. 015,26. 878) ng/L,Z=-7. 981,P=0. 000). CUL4B level in patients was positively correlated with IL?6( r=0. 386,P=0. 008 ) . Conclusion It is of great significance to detect CUL4B and IL?6 in patients with MM on clinical stage,the condition of judgment and efficacy observation.
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Objective To study the inhibitory mechanism of soliquiritigenin on tyrosinase.Methods The inhibition effect and inhibitory kinetics of tyrosinase induced by oliquiritigenin were investigated.The effect of isoliquiritigenin on A375 melanoma cell were preliminarily indicated.Results The inhibitory effect of isoliquiritigenin on monophenolase and diphenolase of tyrosinase was good, IC50 was (11.22 ±0.92) μM and (48.53 ±4.75) μM, isoliquiritigenin was a competitive inhibitor, the value of inhibition constant ( Ki ) was ( 12.14 ±0.54 ) μM.Isoliquiritigenin could inhibit the prolifevation of A375 cell significantly, and the tyrosinase activity and melanin synthesis decreased.Conclusion Isoliquiritigenin as a tyrosinase inhibitor, plays an important role in the regulation of melanin, which provides the theoretical basis for the clinical anti skin cancer.
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Objective To investigate the role of a small RNA interference against VEGF in radiation sensitivity in melanoma .Methods A375 human melanoma cell lines were transplanted into nude mice ,which with malignant melanoma were randomly divided into control group , VEGF negative plasmid group and VEGF positive plasmid group, followed by 4Gy irradiation twice a week for 2 weeks.The volume of tumor was calculated twice a week, the area of tumor necrosis was assayed by HE,the expression of VEGF in tumor was determined by Western-blot and Immunohistochemical. ResuIts The expression of VEGF in VEGF positive plasmid group decreased significantly (P<0.05), VEGF positive group had more tissue necrosis, tumor growth was significantly inhibited (P<0.05).ConcIusion siRNA-VEGF in tumor injection liposome encapsulated in malignant melanoma has a role in the radiation sensitization, which provides an experimental basis for the clinical development of targeted therapy combined with radiotherapy for the treatment of VEGF gene.
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Objective To investigate hospital acquired infections in patients with liver cirrhosis caused by relevant factors.Methods From July 2008 to June 2013,the clinical data of 470 cases of hospital acquired infections patients with chronic hepatitis B virus (HBV) infection were retrospectively analyzed by case-control study for the effective factors.Results Hospital acquired rate was 29.1％ (137/470).By logistic regression analysis,the effective factors were length of stay (OR =27.824,95％ CI 7.187-98.386),invasive operation (OR =17.201,95％ CI 4.245-71.303),a complication (OR =2.138,95％ CI 1.030-4.377),preventive use of antibiotics(OR =2.741,95％CI 1.816-4.010),drinking history(OR=34.248,95％CI 13.045-82.328),serum albumin(OR =17.258,95％ CI 6.242-53.162),quantitative PCR-HBVDNA (OR =4.859,95％ CI 3.214 -7.625),white blood cell (OR =4.271,95 ％ CI 1.520-12.157),c holinesterase (OR =2.761,95 ％ CI 1.523 -3.787) and anti virus medicine(OR=0.128,95％CI 0.041-0.375) (P＜0.05).Conclusion This study shows that length of stay,invasive operation history,complications,low white blood cell,low serum protein,low cholinesterase,the prophylactic use of antibiotics,hormone,high PCR-HBVDNA quantitative and drinking are the important risk of hospital acquired infections infection factors of patients of impact chronic HBV infection.Applications of antiviral drugs are effective in the prevention of chronic HBV infection protection factors of hospital acquired infections infection patients.