RESUMEN
Objective To explore the role of serum bilirubin,homocysteine in the pathogenesis of acute cerebral infarction.Methods 150 patients with cerebral infarction were selected as the study group,and at the same time,110 healthy people were selected as the control group.We used automatic biochemical analyzer to determine the serum bilirubin level and enzyme-linked immunoassay was used to quantitatively determine the plasma homocysteine levels.At last,the data were statistically analyzed.Results (1) The concentrations of total bilirubin,indirect bilirubin and direct bilirubin of the study group were (9.74 ± 3.36) μmoL/L,(5.82 ± 1.65) μmol/L (3.80 ± 1.47) rnol/L,respectively,which were obviously lower than those of the control group [(14.35 ± 4.27) μmol/L,(8.22 ± 2.53) μmol/L,(6.07 ± 2.03) μmol/L,t =-2.528,-2.366,-2.256,all P ＜ 0.05].The concentration of homocysteine of the study group was (22.56 ± 7.35)pmol/L,which was obviously higher than (6.98 ± 3.35)μmol/L of the control group,the difference was statistically significant (t =16.973,P ＜ 0.05).The concentration of homocysteine was related to both total bilirubin(rs =-0.101,P =0.004) and indirect bilirubin (rs =-0.153,P =0.037).But there was no correlation between homocysteine and direct bilirubin (rs =-0.067,P =0.231).Conclusion Homocysteine may be a risk factor for cerebral infarction.Serum bilirubin may be protective factor for cerebral infarction involved in the occurrence and development of cerebral infarction.
RESUMEN
Objective To study the prevalence and sequence homology of virulence genes exoU and exoS in 53 strains of pan-drug-resistant Pseudomonas aeruginosa .Methods The virulence genes exoU and exoS were detected by PCR.Sequence homo-logy was analyzed by BOX-PCR.Results Of the 53 clinical isolates of pandrug-resistant Pseudomonas aeruginosa ,the exoS+/exoU- genotype was identified in 40 strains,exoU+/exoS - genotype in 10 strains,exoS +/exoU+ genotype in 1 strain, and exoS-/exoU- genotype in 2 strains.BOX-PCR results showed that 41 exoS+ isolates belonged to 24 genotypes,and 11 exoU+ strains could be grouped into 7 genotypes.Conclusions The prevalence of virulence genes is high in clinical isolates of pandrug-resistant Pseudomonas aeruginosa .BOX-PCR fingerprint analysis combined with sequence homology analysis is help-ful for effective monitoring and control of hospital pandrug-resistant pseudomonas aeruginosa infection.
RESUMEN
Objective To investigate the resistance and ?-lactamase of cefoxitin-resistant Klebsiella pneumoniae. Methods The minimal inhibitory concentrations were determined by standard agar dilution.Isoelectric focusing was used to measure the PI(s) of an isolate,s ?-lactamase,AmpC and ESBLs activity was confirmed by a three-dimensional extract method. Results The resistant rates of 40 strains were as follows: imipenem and meropenem 0.0%,cefepime 20.6%,cefotaxime(22.5%),ceftazidime 60.0%.The most isolates were demonstrated two or more ?-lactamase bands by IEF.Of 39 strains tested,ESBLs was detected in 31(70.5%) strains and AmpC-type?-lactamase in 16(41.0%) strains by three dimensional extract test. Conclusions These cefoxitin-resistant Klebsiella pneumoniae produced two or more ?-lactamases.It is imperative for clinical microbiology laboratories to detect and research ?-lactamases,especially AmpC enzyme.