Effects of compound ganshao paste on the levels of sex hormones and hepatic transaminases in polycystic ovarian syndrome rat model / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To study the effects of Compound Ganshao Paste (CGP) on the levels of sex hormones and hepatic transaminases in polycystic ovarian syndrome (PCOS) rat model.</p><p><b>METHODS</b>The PCOS rat model was established using Kafali modeling method. Rats were randomly divided into five groups, i. e., the normal control group, the PCOS model group, the Diane-35 group, the Compound Ganshao Caspule group, and the CGP group. The levels of sex hormones were detected using radioimmunoassay. The levels of hepatic transferases were detected using method of enzyme coupling ratio.</p><p><b>RESULTS</b>Compared with the normal control group, the serum levels of luteinizing hormone (LH), testosterone (T), follicle stimulating hormone (FSH), estradiol (E2), and progesterone (P) decreased, showing statistical difference (P<0.05). Compared with the model group, the serum levels of LH, T, and FSH decreased, while serum E2 and P increased in the three medication groups, showing statistical difference (P<0.05). There was insignificant difference in the serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) among the normal control group, the model group, and the CGP group (P>0.05). Compared with the Diane-35 group and the Compound Ganshao Capsule group, the levels of hepatic transaminases of the CGP group was lower with statistical difference (P<0.05).</p><p><b>CONCLUSIONS</b>CGP could improve the ovarian functions through adjusting the endocrine functions of PCOS model rats, thus stimulating the follicular development and ovulation. CGP did not add the hepatic burden.</p>

Clinical observation on effect of Tiaomian Huayu Decoction in treating endometriosis / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To investigate the clinical efficacy of Tiaomian Huayu Decoction (THD) in treating patients with endometriosis.</p><p><b>METHODS</b>Patients in the treated group were administered with THD, those in the control group were given provera. T-cell sub-population including CD3, CD4, CD8 and CD4/CD8 ratio, and natural killer (NK) cell in peripheral blood in the two groups before and after treatment were determined.</p><p><b>RESULTS</b>Among the 66 patients in the treated group, the treatment results was cured in 10, significantly effective in 22, effective in 25 and ineffective in 9, the total effective rate was 86.4%, while of the 30 in the control group, the corresponding number was 2, 6, 12, 10 and 66.7%, which was significantly different from that in the treated group respectively (P<0.05). The levels of CD4 and CD4/CD8 ratio were higher, CD8 and NK were lower in the treated group than those in the normal group before treatment (P < 0.01), while these indexes were restored to some extent but still did not reach the normal level after treatment. All the above-mentioned indexes in the control group were insignificantly changed after treatment (P >0.05).</p><p><b>CONCLUSION</b>THD has definite effect in treating endometriosis, and it can also regulate the immune function of patients.</p>

Clinical study of ganshao capsule in treating clomiphene-resistant polycystic ovarian syndrome / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To assess the efficacy and safety of Ganshao Capsule (GSC) in treating clomiphene (CC)-resistant hyperandrogenemic polycystic ovarian syndrome (PCOS), and to explore the feasibility of using CC for improving ovulation induction after withdrawal of GSC.</p><p><b>METHODS</b>Twenty-seven PCOS patients were given GSC for 8 weeks (2 cycles). Changes in serum reproductive hormone, body mass index (BMI), adverse reaction, as well as pelvic ultrasonographic feature were observed before treatment, at the end of 4 weeks and 8 weeks after treatment. After stopping GSC medication, CC was used to induce ovulation in patients whose serum testosterone (T) < (2.1+/-0.8) nmol/L but without ovulation. Condition of follicle growth and effect of GSC were monitored.</p><p><b>RESULTS</b>After 4 weeks of treatment, serum reproductive hormones were significantly changed. At the end of 8 weeks, bilateral ovarian volume, number of follicles, and diameter of follicles were significantly reduced, and endometrium obviously thinned. Within 2 months after stopping medication, 6 in the 27 patients got natural ovulation, and 2 natural pregnancy. In the other 19 patients who received ovulation induction during the 37th cycles, 17 had ovulation in the 25th cycles, 7 got pregnancy, the ovulation rate being 89.5%, ovulation cycle rate 67.6%, pregnant rate 36.8%, and pregnant cycle rate 28.0%.</p><p><b>CONCLUSION</b>Endometrium, ovarian morphology and BMI got significant improvement in PCOS patients with CC- resistance and hyperandrogenemia. The sensitivity to ovulation induction with CC were also improved.</p>