Chemical constituents from dried sorophore of cultured Cordyceps militaris / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the chemical constituents of the dried sorophore of cultured Cordyceps militaris.</p><p><b>METHOD</b>Compounds were isolated and purified by macroporous adsorption resin and silica gel column chromatography. Their chemical structures were elucidated on the basis of physicochemical properties and spectral data (IR, FAB-MS, 1H-NMR and 13C-NMR).</p><p><b>RESULT</b>Nine compounds were isolated and identified as: ergosta-4, 6, 8 (14)-tetraen-3-one (1), citrostadienol (2), tetracosanoic acid 2, 3-dihydroxypropyl ester (3), ergosterol (4), ergosterol peroxide (5), ergosta-7, 22-dien-3beta, 5alpha, 6beta-triol (6), cordycepin (7), adenosine (8), N-(2-hydroxyethyl) adenosine (9), respectively.</p><p><b>CONCLUSION</b>Compounds 1-3, 6, 9 were separated from the sorophore of cultured C. militaris for the first time.</p>

Alkaline-degradation products of ginsenosides from leaves and stems of Panax quinquefolium / 药学学报

<p><b>AIM</b>To study the alkaline-degradation products of ginsenosides from leaves and stems of Panax quinquefolium L.</p><p><b>METHODS</b>Isolation and purification were carried out on silica gel and HPLC; the structures of chemical constituents were elucidated by spectral analysis.</p><p><b>RESULTS</b>From the alkaline-degradation products, nine compounds were identified as: 20 (S) -protopanaxadiol (I), 20 (S) -dammar-25 (26)-ene-3beta, 12beta, 20-triol (II), 24 (R) -ocotillol (III), 20 (S) -protopanaxatriol (IV), 20 (S) -dammar-25 (26)-ene-3beta, 6alpha, 12beta, 20-tetrol (V), dammar-20 (21), 24-diene-3beta, 12beta-diol (VI), dammar-20(21), 24-diene-3beta, 6alpha, 12beta-triol (VII), 20 (S), 24 (S) -dammar-25 (26) -ene-3beta, 6alpha, 12beta, 20, 24-pentanol (VIII), 20 (S) -dammar-23-ene-25-hydroperoxyl-3beta, 6alpha, 12beta, 20-tetrol (IX).</p><p><b>CONCLUSION</b>The configuration of C20 position of ginsenosides was not changed by alkaline-degradation. The complete assignments of 1H and 13C NMR chemical shifts of four new compounds V, VII, VIII, IX, were acquired by means of 2D NMR spectra. Compound I showed antitumor effect on human colon carcinoma cells in vitro.</p>