Closed reduction and three Kirschner pin fixation at the radial side for the treatment of supracondylar fracture in children with Gartland type II and type III / 中国骨伤

<p><b>OBJECTIVE</b>To investigate the characteristics of supracondylar fracture of humerus in children and to explore the effect of closed reduction and internal fixation at radial side on the fracture.</p><p><b>METHODS</b>The children with fractures of Gartland type II and type III in our hospital from June 2010 to June 2013 were reviewed. There were 28 males and 7 females, ranging in age from 1 year and 1 month to 2 years and 6 months, with an average of 2 years and 1 month. According to Gartland classification, 19 cases were type II, 16 cases were type III. There were 3 patients with radial nerve injuries, and 5 patients with anterior interosseous nerve injuries. There were no vascular injuries. All the patients were treated with closed reduction and three Kirschner fixation at the radial side, followed by the plaster external fixation with elbowed flexion at 90 °. The X-ray examination was performed at the second day after operation. The joint function exercise began about at 2 to 3 weeks after operation when the plaster fixation was removed, and opportune time for removal of Kirschners depends on the situation of fracture healing. The operation time, nerve recovery, and the elbow joint function were observed.</p><p><b>RESULTS</b>All the children were followed up, and all the fractures had bony union. According to Flynn score system at the final follow-up, 28 patients got an excellent result, 4 good, 1 poor and 2 bad.</p><p><b>CONCLUSION</b>Three Kirschner fixation at the radial side for the treatment of supracondylar fracture of humerus has advantages of minimally invasive, shorter time of hospitalization, simple removal of the internal fixation and reliable therapeutic effects.</p>

Expression of SOX9 and COL2A1 gene in human cervical intervertebral disc nucleus pulpusus and its significance / 第二军医大学学报

Objective: To observe the expression of SRYCsex determining region Y)-box 9 (SOX9) and collagen type E alpha 1 (COL2A1) in human cervical intervertebral disc nucleus pulposus (NP) tissues of various degeneration degrees, so as to discuss the role of their expression during cervical intervertebral disc degeneration(CIDD). Methods: Totally 36 human cervical intervertebral disc specimens and their pre-operation MRI findings were obtained from surgical patients or patients with cervical trauma, and they were classified into five grades according to the Miyazaki grading system. The expression of SOX9 and COL2A1 was examined by fluorescence quantitative RT-PCR, Western blotting analysis and immunohistochemistry staining. The relationship between SOX9, COL2A1 expression and the degrees of CIDD was analyzed, and the relationship between SOX9 and COL2A1 was also discussed. Results: Both SOX9 and COL2A1 were expressed in NP tissues, with SOX9 seen in the nucleus and COL2A1 in the extracellular matrix. The expression of SOX9 and COL2A1 mRNA and protein gradually decreased as Miyazaki I progressed to Miyazaki V. We also found a linear relationship between COL2A1 (Y) and SOX9 (X) expression: Y=0. 923X-0. 059 (P<0. 001). Conclusion: The expression of SOX9 and COL2A1 mRNA decrease with the increase of CIDD severity, with SOX9 and COL2A1 mRNA having a similar decreasing tendency. SOX9 positively regulate COL2A1 expression, indicating that changes of SOX9 and COL2A1 gene might be one of the molecular mechanisms of CIDD.

Single nucleotide polymorphism of genes associated with intervertebral disc degeneration: Recent progress / 第二军医大学学报

Genetic susceptibility exists in intervertebral disc degeneration (IDD) diseases, which may be associated with gene polymorphisms. Recently, much progress has been made in the relationship between IDD susceptibliity and gene single nucleotide polymorphism(SNP). The genes associated with IDD manly include 3 types: genes associated with intervertebral disc stability (about structure and metabolism), genes associated with inflammation, and genes associated with pain signal channel. Studies of SNP in genes associated with IDD can Hp to identify its molecular mechanism, paving a way for individualized prevention and treatment of IDD.