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Journal of Biomedical Engineering ; (6): 519-523, 2012.
Artículo en Chino | WPRIM | ID: wpr-271741

RESUMEN

This paper is aimed to investigate the transcription and expression of BCR-ABL-pIRES-SEA fusion gene vaccines in vivo in mice. The reconstructed plasmids (BCR-ABL-pIRES-SEA) which were developed previously in our laboratory were injected into the skeletal muscles of BALB/c mice at 14d intervals for three cycles. The transcription and expression of BCR-ABL and staphylococcal enterotoxin A (SEA) in injection site were detected using RT-PCR and immunohistological methods. The BCR-ABL/SEA mRNA and protein could be identified in the injection site of BCR-ABL-pIRES-SEA vaccinated mice. The reconstructed BCR-ABL-pIRES-SEA plasmids can effectively express gene production in the skeletal muscles of mice and have the common features of DNA vaccine.


Asunto(s)
Animales , Masculino , Ratones , Enterotoxinas , Genética , Alergia e Inmunología , Metabolismo , Proteínas de Fusión bcr-abl , Genética , Alergia e Inmunología , Metabolismo , Ratones Endogámicos BALB C , Músculo Esquelético , Metabolismo , Plásmidos , Alergia e Inmunología , ARN Mensajero , Genética , Metabolismo , Proteínas Recombinantes de Fusión , Genética , Alergia e Inmunología , Metabolismo , Vacunas de ADN , Alergia e Inmunología
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