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Objective To observe the therapeutic effect of sinomenine(SN) in adjuvant induce arthritis(AIA) model rats and its influence on inflammation related factors expressions.Methods The AIA rat model was duplicated by adopting complete Freund's adjuvant(FCA) induction method.After successfully constructing the model,the rats were randomly divided into the model group,methotrexate group,SN low,middle and high dose groups.The rats without constructing model were taken as the normal control group.Taking materials was performed after 21 d continuous medication gavage.The rat joint injury was observed by HE staining and the pathological semiquantitative scoring was performed.The serum levels of rheumatoid factor(RF),C-reactive protein (CRP),interleukin-1(IL-1),interleukin-6(IL-6),and interleukin-10(IL-10) were tested by using the automatic blood biochemical analyzer.Results Comparing with the model group,the joint injury degree in the methotrexate group,SN low and middle dose groups were obviously mild and the pathological semiquantitative score was significantly decreased(P＜0.05),serum CRP level was significantly deceased(P＜0.05),the serum inflammation related factors IL-1 and IL-6 levels were significantly decreased,while the IL-10 level was significantly increased (P＜0.01).Conclusion Low and middle dose SN can regulate the imbalance of proinflammatory factors and inflammation-suppressing factors expression levels,thus significantly improve the joint pathological injury degree in rheumatoid arthritis.
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Objective To provide a theoretical basis for the pulmonary enteric therapy in treating intestinal dysbacteriosis complicating allergic asthma according to the traditional theory of Lung and Large intestine are interiorly-exteriorly.Methods The experiment included the blank group,model group,positive control group and three kinds of traditional Chinese medicine compound administration group;the intestinal dysbacteriosis in SD rats was duplicated by adopting cefoperazone and candida albicans gavage.On this basis ovalbumin injection sensitization and nebulization were applied to induce asthma.Meanwhile Jia-Wei-ShengJiang Powder(SJS),Guizhi-Jia-Houpu-Xingzi Decoction(GHX) and Zeng-Ye-Cheng-Qi Decoction(ZYCQ) were orally administered for treatment of lung-intestine combine therapy,lung therapy and intestine therapy.The respiratory function was analyzed by using the Buxco whole body plethysmography.The variety of intestinal flora were analyzed by using 16SrDNA.Results Analyzing the specieces composition of intestinal flora in each group by the OUT value found that the recovery of Lactobacillus in each group was close to that in the blank group and better than that in the positive group,meanwhile,3 treatment groups all could promote the recovery of Proteobacteria and bacteroidetes,but the lung-intestine combine therapy had optimal effect,the Alpha diversity analysis found that the intestinal microflora diversities from high to low were in turn the intestinal therapy group,lung therapy group,lungintestine combined therapy group and model group;the respiratory frequency and airway hyperreactivity in the model group was 260.56(1.28),which in the lung-intestine combined therapy group,lung therapy and intestine therapy after treatment were 141.79 (0.93),137.25 (1.30) and 203.16 (0.84) respectively,the rat respiratory frequency in each treatment group was lower than that in the model group,while the airway hyperreactivity only in the lung-intestine combined therapy group and intestinal therapy group was lower than that in the model group,moreover showing statistical significance.Conclusion The lung-intestine combine therapy method for treating rat intestinal dysbacteriosis complicating allergic asthma is superior to simple lung therapy method or intestine therapy method.
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Objective To study the effect of extract of cockroach on animals with acute hepatic injury and its antiviral action in vitro. Methods The animal models with hepar injury were established by CCl4, bcg vaccine and lipopolysaccharide (LPS) . And the activities of ALT and AST in serum were measured. Serum of ducks was separated after cockroach extract was administrated. The serum was added to the culture fluid of HBV-DNA transfected hepatic cancer cells-HepG2. Results After the cockroach extract was administrated, the activities of ALT and AST in mouse serum were reduced. Both the therapeutic indexes of cockroach extract and lamivudine were larger than 2. Conclusions Cockroach extract can improve the liver function of animals with acute hepatic injury, and inhibit the secretion of ALT and AST in serum.