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<p><b>BACKGROUND</b>Surgical resection becomes standard treatment for pulmonary metastatic tumor with operative indication. The aim of this study is to analyze the indication and surgical efficacy of pulmonary metastasectomy.</p><p><b>METHODS</b>From November 1991 to May 2003, a total of 26 patients with pulmonary metastatic tumors were treated surgically in our hospital, 12 cases were males and 14 females, with an average age of 50 years old. The metastatic tumors came from different organs and tissues, 5 cases accompanied by metastatic tumors of liver or brain.</p><p><b>RESULTS</b>Four cases underwent pneumonectomy, 8 lobectomy, and 14 wedge resection (in which 8 cases underwent mildly invasive operation using video-assisted thoracic surgery). No operative death occurred. 5-year survival rate was 27.3% (3/11), 2-year survival rate 42.9% (9/21) and 1-year survival rate 69.2% (18/26).</p><p><b>CONCLUSIONS</b>Pulmonary metastasectomy is recommended in pulmonary metastases with operative indication, and is still effective in cases accompanied by resectable metastatic tumors of liver or brain. Mildly invasive operation using video-assisted thoracic surgery might be chosen for some cases with single metastatic locus.</p>
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Self-made molecule imprinting polymer-solid phase extraction column was used in extracting nerve agent degradation products in rice. The extraction was then examined by capillary electrophoresis. The method was simple, reliable and sensitive. The calibration curve showed a good linearity for the nerve agent degradation products in rice was in the concentration range of 0.2～5.0 μg/g and the detection limits were 0.05μg/g. The RSD of the method was less than 6.2%.
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Objective To observe the inhibition of asON phosphorothioate to the TIMP-1 gene and protein expression in the liver tissue of immune- induced hepatic fibrosis rats. Methods According to the analysis of modulator, structure protein, encoding sequence of TIMP-1 genome, we designed four different groups of asONs. These asONs were injected into the hepatic fibrosis rat models through coccygeal vein. The results were observed by RT-PCR, immunohistochemistry and in situ hybridization with collagen Ⅰ、Ⅲ, special staining of collagen fiber, electron microscope. Results The asON phosphorothioate of TIMP-1 could be expressed in vivo, and could block the TIMP-1 gene and protein expression in the liver of immune- induced hepatic fibrosis rats on the level of mRNA, which could promote the degradation of collagen Ⅰ、Ⅲ(P
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An experimental immunity hepatic fibrosis rat model was prepared by means of immunologic assault with human serum albumin, and normal rats served as a control group. The immunohistochemistry methods and in situ hybridization were respectively used to detect TIMP 2 mRNA and related antigens in the liver,and to investigate the localization and expression of TIMP 2 in the liver of both normal and experimental hepatic fibrosis rats. The results showed that TIMP 2 mRNA and related antigens in the livers of experimental group were expressed in myofibroblasts and fibroblasts, especially in the portal area and fibrous septum. The positive signal was located in the cytoplasm, but not in the nucleus. On the other hand, there was a high level of expression of TIMP 2 in the liver of the experimental group.It is suggested that in the process of hepatic fibrosis, fibroblasts and myofibroblasts are the major cells expressing TIMP 2. The severer the hepatic fibrosis in the injured liver is, the higher the levels of TIMP 2 related antigens and gene expression are.