Evaluation of silybum marinaum efficacy on University of Wisconsin and histidine-tryptophan-ketoglutarate solutions latter the damage of the perfused liver

Abstract Purpose: To investigate the hepatoprotective and antioxidant effeicacies of Silybum marianum's (silymarin, S) on University of Wisconsin (UW) and histidinetryptophan-ketoglutarate (HTK) preservation solutions. Methods: Thirty two Wistar albino adult male rats were used. Group 1: UW group, Group 2: UW + Silymarin group(S), Group 3: HTK group, Group 4: HTK + silymarin group (S), respectively. Silymarin was enforced intraperitoneally before the surgery. Biopsies were enforced in 0, 6 and 12.hours to investigate. Results: Biochemical parameters examined in alanine aminotransferase (ALT), furthermore superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) in rats were also evaluated. Detected histopathological changings were substantially declining in the groups that received silymarin, cellular damage was decreased significantly in HTK + Silymarin group, according to other groups. It has been identified as the most effective group was HTK + silymarin group in evaluation of ALT, electron microscopic results, also decreased MDA and elevated in SOD, and CAT activity. Caspase 3 analysis showed a substantial lower apoptosis ratio in the silymarin groups than in the non-performed groups (p<0.05). Conclusion: Histidinetryptophan-ketoglutarate+silymarin group provides better hepatoprotection than other groups, by decreasing the hepatic pathologic damage, delayed changes that arise under cold ischemic terms.

Laparoscopic partial cystectomy for the treatment of hepatic hydatid cysts.

Background: The laparoscopic approach for the treatment of hepatic hydatid cysts is increasingly gaining importance. The aim of this study was to report a series of 27 patients with hepatic hydatid cyst managed with laparoscopic partial cystectomy. Methods: A retrospective review of patients treated at a university clinic for hepatic hydatid cysts from March 2010 to May 2014 was performed. Operative time, blood loss, length of hospital stay, post-operative complications, and early follow-up outcomes were evaluated. Results: Laparoscopic surgical intervention was performed on 27 patients (17 females and 10 males)who were diagnosed with hydatid cysts by ultrasonography and computed tomography (CT). Except 3 of the cysts, were located in the right lobe of the liver. No mortality was noted during the study. Two surgical site infections were observed and no abscesses developed in the cystic cavity. Recurrence was not noted during the mean follow-up Period of 22 months. Conclusion: Laparoscopic partial cystectomy is a safe and effective method for the treatment of hepatic hydatid cyst.