Effect of Tributyltin Compound on \it{N}-methyl-\it{D}-aspartate (NMDA) Receptors in Brain of Preweanling Mice

Objective: The aim of this study was to investigate the effect of tributyltin (TBT) compound on N-methyl-D-aspartate (NMDA) receptors in the brains of preweanling mice. Methods: Pregnant ICR mice were exposed to TBT chloride at concentrations of 0, 15, and 50 ppm in water. Male offspring were sacrificed at 1, 2 and 3 weeks after birth. Mouse brain membranes were prepared from cerebral cortices, and the specific binding of [3H]MK-801 to an NMDA receptor was determined by radioligand binding assay. Results: The mean body weight of preweanling mice of the 50 ppm dose group decreased by 17-25% (p<0.01) at 1, 2 and 3 weeks of age, compared with that of preweanling mice of the corresponding control group. The [3H]MK-801 binding level significantly decreased (p<0.05) in the 15 ppm F1 group at 1 week and in the 15 ppm and 50 ppm F1 groups at 3 weeks of age, compared with that in the corresponding control F1 group. Conclusions: The exposure to TBT via placenta and dam’s milk seriously affected not only the growth of preweanling mice, but also the F1 cerebral NMDA receptors involved in memory and learning.

Effect of tributyltin compound onN-methyl-D-aspartate (NMDA) receptors in brain of preweanling mice

<p><b>OBJECTIVE</b>The aim of this study was to investigate the effect of tributyltin (TBT) compound onN-methyl-D: -aspartate (NMDA) receptors in the brains of preweanling mice.</p><p><b>METHODS</b>Pregnant ICR mice were exposed to TBT chloride at concentrations of 0, 15, and 50 ppm in water. Male offspring were sacrificed at 1, 2 and 3 weeks after birth. Mouse brain membranes were prepared from cerebral cortices, and the specific binding of [(3)H]MK-801 to an NMDA receptor was determined by radioligand binding assay.</p><p><b>RESULTS</b>The mean body weight of preweanling mice of the 50 ppm dose group decreased by 17-25% (p<0.01) at 1, 2 and 3 weeks of age, compared with that of preweanling mice of the corresponding control group. The [(3)H]MK-801 binding level significantly decreased (p<0.05) in the 15 ppm F1 group at 1 week and in the 15 ppm and 50 ppm F1 groups at 3 weeks of age, compared with that in the corresponding control F1 group.</p><p><b>CONCLUSIONS</b>The exposure to TBT via placenta and dam's milk seriously affected not only the growth of preweanling mice, but also the F1 cerebral NMDA receptors involved in memory and learning.</p>