Effect of Alpinia officinarum Hance alcohol extracts on primary dysmenorrheal

Objective To study the effect of Alpinia officinarum Hance (A. officinarum) 80% alcohol extract on the primary dysmenorrhea. Methods A. officinarum 80% alcohol extract were enriched by macroporous adsorption resins. Female mice of primary dysmenorrhea model were established by oxytocin induction; the effects of A. officinarum 80% alcohol extract on primary dysmenorrhea were observed by body twist method; and the homogenate level of prostaglandin F

Effect of Alpinia officinarum Hance alcohol extracts on primary dysmenorrheal

OBJECTIVE@#To study the effect of Alpinia officinarum Hance (A. officinarum) 80% alcohol extract on the primary dysmenorrhea.@*METHODS@#A. officinarum 80% alcohol extract were enriched by macroporous adsorption resins. Female mice of primary dysmenorrhea model were established by oxytocin induction; the effects of A. officinarum 80% alcohol extract on primary dysmenorrhea were observed by body twist method; and the homogenate level of prostaglandin F2α (PGF2α), prostaglandin E2 (PGE2) and Ca(2+) in the uterus were observed in oxytocin-induced female mice.@*RESULTS@#The writhing frequency of primary dysmenorrhea mice was significantly decreased after treatment of A. officinarum 80% alcohol extract and the level of PGF2α, PGE2 and Ca(2+) in mice uterus was significantly decreased (P < 0.05, P < 0.01) in groups of mice treated with middle and high dosage of A. officinarum 80% alcohol extract compared with that of model group.@*CONCLUSIONS@#These findings suggest that A. Officinarum 80% alcohol extract can significantly relieve primary dysmenorrhea.

Matrix metalloproteinase-9 was involved in the immuno-modulatory defect of mesenchymal stem cell from chronic myeloid leukemia patients / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Overwhelming evidences on chronic myeloid leukemia (CML) indicate that patients harbor quiescent CML stem cells that are responsible for blast crisis. While the hematopoietic stem cell (HSC) origin of CML was first suggested over 30 years ago, recently CML-initiating cells beyond HSCs are also being investigated.</p><p><b>METHODS</b>We have previously isolated fetal liver kinase-1-positive (Flk1(+)) cells carrying the BCR/ABL fusion gene from the bone marrow of Ph(+) patients with hemangioblast property. In this study, we isolated CML patient-derived Flk1(+)CD31(-)CD34(-) mesenchymal stem cells (MSCs) and detected their biological characteristics and immunological regulation using fluorescence in situ hybridization (FISH) analysis, fluorescence activated cell sorting (FACS), enzyme-linked immunoadsorbent assay, mixed lymphocyte reaction assays; then we compared these characters with those of the healthy donors.</p><p><b>RESULTS</b>CML patient-derived Flk1(+)CD31(-)CD34(-) MSCs had normal morphology, phenotype and karyotype while appeared impaired in immuno-modulatory function. The capacity of patient Flk1(+)CD31(-)CD34(-) MSCs to inhibit T lymphocyte activation and proliferation was impaired in vitro.</p><p><b>CONCLUSIONS</b>CML patient-derived MSCs have impaired immuno-modulatory functions, suggesting that the dysregulation of hematopoiesis and immune response may originate from MSCs rather than hematopoietic stem cells (HSCs). MSCs might be a potential target for developing efficacious treatment for CML.</p>

A new phenolic glycoside from prunella vulgaris / 药学学报

A new phenolic glycoside was isolated from the spikes of Prunella vulgaris. Its structure was elucidated as gentisic acid 5-O-beta-D-(6'-salicylyl)-glucopyranoside by spectroscopic evidence and chemical analysis.

Chemical constituents from Hedyotis diffusa / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the chemical constituents from Hedyotis diffusa.</p><p><b>METHOD</b>The compounds were isolated and purified by various chromatographic techniques and identified by their physicochemical properties and spectral data.</p><p><b>RESULT</b>Eight compounds were isolated and identified as octadecyl (E)-p-coumarate (1), p-E-methoxy-cinnamic acid (2), ferulic acid (3), scopoletin (4), succinic acid (5), aurantiamide acetate (6), rubiadin (7), robustaquinone D (8).</p><p><b>CONCLUSION</b>Compounds 1-8 were obtained from genus Hedyotis for the first time.</p>

Studies on chemical constituents of Prunella vulgaris / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Prunella vulgaris.</p><p><b>METHOD</b>To separate the constituents of P. vulgaris by using various kinds of chromatography and identify their structures on the basis of spectral analysis.</p><p><b>RESULT</b>Seven compounds were isolated from the spikes of P. vulgaris. Their structures were established as autantiamide acetate (1), rhein (2), tanshinone I (3), danshensu (4), stigmast-7, 22-dien-3-one (5), 3, 4, alpha-trihydroxy-methyl phenylpropionate (6), butyl rosmarinate (7).</p><p><b>CONCLUSION</b>Compounds 1-4 were isolated from this genus for the first time.</p>

Chemical constituents from roots Pseudostellaria heterophylla / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Pseudostellaria heterophylla.</p><p><b>METHOD</b>The compounds were isolated by silica gel and Sephadex LH-20 column chromatography and purified by recrystallization. Their structures were elucidated on the basis of physicochemical properties and spectral analysis.</p><p><b>RESULT</b>Seven compounds were identified as 2-minalin (1) , 3-furfuryl pyrrole-2-carboxylate (2), ursolic acid (3), acacetin (4), luteolin (5), acacetin 7-O-beta-D-glucopyranosyl (6-->1)-alpha-L-rhamnopyranoside (6).</p><p><b>CONCLUSION</b>Among them, compounds 3-6 were isolated from the plant for the first time.</p>

A new alkaloid from Salsola collina / 药学学报

Salsola collina is widely distributed in droughty and semi-droughty area, which is used as a kind of folk remedy in traditional Chinese medicine for treatment of hypertension. The study is on the chemical constituents of this herb from its aerial parts to obtain its active constituents. Dried and crushed aerial parts of this herb were extracted three times with 95% EtOH at reflux. The ethanol extracts were combined and concentrated under reduced pressure at 70 degrees C to yield residue, which was suspended in water and successively partitioned with light petroleum, chloroform and n-butanol. The chloroform and n-butanol fractions were treated by various chromatographic techniques, such as silica gel, C18 reversed-phase silica gel and macroporous resin column chromatography. Compounds were elucidated by their physicochemical properties and spectroscopic analysis. In the course of our study on searching biological active components from this herb, a new alkaloid together with three known alkaloids were isolated and identified as N-transferuloyl-3-methyldopamine (1), 3-[4-(beta-D-glucopyranosyloxy)-3-methoxyphenyl]-N-[2-(4-hydroxyl-3-methoxyphenyl) ethyl]-2-propenamide (2), salsoline A (3), salsoline B (4). Compound 4 is a new compound and named as salsoline B, while compound 2 was obtained in Salsola collina for the first time.

Studies on chemical constituents of Salsola collina / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Salsola collina.</p><p><b>METHOD</b>The EtOH extract from the whole plant of S. collina were isolated and purified by column chromatography. These compounds were identified by their physical properties and spectroscopic data.</p><p><b>RESULT</b>Eleven compounds were isolated and identified as ferulic acid (1), p-coumaric acid (2), salicylic acid (3), tricin (4), selagin (5), acanthoside D (6), tricin -7-O-beta-D-glucopyranoside (7), tricin-4'-O-beta-D-apioside (8), isorhamnetin-7-O-beta-D-glucopyranoside (9), isorhamnetin-3-O-beta-D-glucopyranoside (10), isorhamnetin-3-O-alpha-L-arabinopyranosyl (1-->6) -beta-D-glucopyranoside (11).</p><p><b>CONCLUSION</b>wherein compound 1, 2, 3, 5, 6 and 9 were isolated from the Salsola for the first time.</p>

Studies on chemical constituents in root tuber of Cynanchum auriculatum / 中国中药杂志

<p><b>OBJECTIVE</b>To study chemical constituents from Cynanchum auriculatum.</p><p><b>METHOD</b>The chemical components were isolated and purified by silca gel, sephedex-LH-20 and ODS column chromatography. The chemical structures were elucidated on the basis of physicochemical properties and spectral data.</p><p><b>RESULT</b>Thirteen compounds were isolated and identified as: caudatin (1), metaplexigenin (2), cynauricuoside A (3), succinic acid (4), azelaic acid (5), wilforibiose (6), sucrose (7), 1-O-hexadecanolenin (8), beta-amyrin acetate (9), cynanchone A (10), acetylquinol (11), beta-sitosterol (12), daucosterol (13).</p><p><b>CONCLUSION</b>Compounds 4-9 were obtained from this plant for the first time.</p>

Isolation and characterization of phenylethanoid glycosides from Clerodendron bungei / 药学学报

<p><b>AIM</b>To study the chemical constituents from Clerodendron bungei Steud.</p><p><b>METHODS</b>The compounds were isolated and purified by various chromatographic techniques and identified by their physicochemical properties and spectral data.</p><p><b>RESULTS</b>Ten phenylethanoid glycosides were isolated and identified as clerodendronoside (1), acteoside (2), isoacteoside (3), cistanoside C (4), jionoside C (5), leucosceptoside A (6), cistanoside D (7), campneoside I (8), campneoside II (9), cistanoside F (10).</p><p><b>CONCLUSION</b>Compound 1 is a new phenylethanoid glycoside, while compounds 4-10 are obtained from this plant for the first time.</p>