WNT7A promotes tumorigenesis of head and neck squamous cell carcinoma via activating FZD7/JAK1/STAT3 signaling / 国际口腔科学杂志·英文版

Wnt signaling are critical pathway involved in organ development, tumorigenesis, and cancer progression. WNT7A, a member of the Wnt family, remains poorly understood in terms of its role and the underlying molecular mechanisms it entails in head and neck squamous cell carcinoma (HNSCC). According to the Cancer Genome Atlas (TCGA), transcriptome sequencing data of HNSCC, the expression level of WNT7A in tumors was found to be higher than in adjacent normal tissues, which was validated using Real-time RT-PCR and immunohistochemistry. Unexpectedly, overexpression of WNT7A did not activate the canonical Wnt-β-catenin pathway in HNSCC. Instead, our findings suggested that WNT7A potentially activated the FZD7/JAK1/STAT3 signaling pathway, leading to enhanced cell proliferation, self-renewal, and resistance to apoptosis. Furthermore, in a patient-derived xenograft (PDX) tumor model, high expression of WNT7A and phosphorylated STAT3 was observed, which positively correlated with tumor progression. These findings underscore the significance of WNT7A in HNSCC progression and propose the targeting of key molecules within the FZD7/JAK1/STAT3 pathway as a promising strategy for precise treatment of HNSCC.

Expression and significance of programmed death-1 and T-cell immunoglobulin- and mucin domain-3-containing molecule 3 in hepatocellular carcinoma / 临床肝胆病杂志

ObjectiveTo investigate the expression and clinical significance of programmed death-1 (PD-1) and T-cell immunoglobulin- and mucin domain-3-containing molecule 3 (TIM-3) in hepatocellular carcinoma (HCC). MethodsThe paraffin blocks of HCC tissue and adjacent tissue (＞1 cm from the tumor margin) were collected from 46 patients with HCC who underwent surgical treatment in No. 900 Hospital of The Joint Service and Security Force of The Chinese People’s Liberation Army from January 2013 to December 2015. Immunohistochemistry was used to measure the expression of PD-1 and TIM-3, and their association with clinicopathological features and prognosis was analyzed. The Wilcoxon test was used for comparison of paired ranked data, and a Spearman correlation analysis was used to investigate the correlation between ranked data; the Kaplan-Meier method was used for survival analysis, and the log-rank test was used for comparison of survival rates between groups; the Cox regression model was used to perform a multivariate analysis. ResultsThe expression levels of PD-1 and TIM-3 in HCC tissue were higher than those in adjacent tissue (both P＜0.05). PD-1 was mainly localized in lymphocytes and TIM-3 was mainly localized in tumor-associated macrophages. The expression of PD-1 was positively correlated with that of TIM-3 in HCC tissue (rs=0.397, P=0.006). The expression level of PD-1 in HCC tissue was associated with tumor size (rs=0.480, P=0.001), portal vein tumor thrombus (rs=0.307, P=0.038), and TNM stage (rs=0.534, P＜0.001), and the expression level of TIM-3 was associated with portal vein tumor thrombus (rs=0.301, P=0.042), degree of pathological differentiation (rs=0356, P=0.015), and TNM stage (rs=0.416, P=0.004). For the patients with HCC, the 1-, 3-, and 5-year overall survival rates were 89.1%, 56.5%, and 34.4%, respectively, and the 1-, 3-, and 5-year disease-free survival rates were 52.2%, 21.7%, and 10.9%, respectively. The multivariate analysis showed that degree of pathological differentiation (hazard ratio ［HR］=4.723, 95% confidence interval ［CI］: 1.618-7.684, P=0.001), tumor size (HR=3.234, 95%CI: 1.327-7.883, P=0.010), TNM stage (HR=3.254, 95%CI: 1.076-9.835, P=0.037), and expression level of TIM-3 (HR=0.572, 95%CI: 0.329-0.995, P=0048) were independent prognostic factors for the overall survival of HCC patients, and degree of pathological differentiation (HR=2.945, 95%CI: 1.527-5.682, P=0.001) and TNM stage (HR=2.074, 95%CI: 1.259-9.793, P=0.016) were independent prognostic factors for the disease-free survival of HCC patients. ConclusionHigh expression levels of both PD-1 and TIM-3 are observed in HCC tissue, and PD-1 and TIM-3 may be involved in the progression and poor prognosis of HCC patients.

Compare the influence of two explorers on the enamel of artificial initial caries / 实用口腔医学杂志

Objective: To compare the influence of two explorers on the enamel of artificial initial caries. Methods: Built an artificial initial caries model in vitro. Compared the influence of two explorers on the enamel of artificial initial caries. Results: 1.The holes probed by sharp explorer were deep and narrow. The holes probed by CPI explorer were shallow and wide. 2. The average strain energy produced by CPI explorer was larger than sharp explorer(Z=-2.021,P=0.043), But there was no significant difference because the strain energy produced by two explorers were in the same rate range. Conclusion: The damage on the enamel of artificial initial caries caused by sharp explorer is not larger than that caused by CPI explorer. So we suggest that the sharp explorer can still be used in sampling survey and clinical examination. But a gentle probing action is strongly recommended.

Marginal microleakage in class Ⅱ-restorations after use of targis inlay / 中华医学美学美容杂志

Objective To evaluate the marginal microleakage in class Ⅱ-restorations after use of targis inlay. Methods Ten extracted human molar teeth were prepared for MO(mesio-occlusal) and DO(disto-occlsa) cavities. In one type of cavity gingival margin was placed above the cemento-enamel junction (CEJ). In another type gingival margin was placed below the CEJ. Cavities were filled with targis inlays. All teeth were thermocycled 200 times between 0℃ and 56℃. Finally, all teeth were cut in mesial-distal direction with a band saw and the numbers of walls of cavities were determined under scanning electron microscope. Results There was no any microleakage in occlusal margin. The number of inlay microleakage of gingival margin placed below CEJ (30 %) was more than that of gingival margin placed above the CET (10 %) ( P

Comparison of two kinds of probes examinations for caries in the sampling survey of 12-year-old children in Fujian province / 实用口腔医学杂志

Objective:To understand the prevalence of caries in the children aged 12 years in Fujian Province and to compare the influence of two kinds of dental probes examinations on caries detect rate.Methods:Based on oral health investigation of WHO and national oral health survey standards,multi-stage stratified sampling was used to randomly select 792 children in Fujian province to accept examination separately by two dental probes.Results:The prevalence of dental caries of youth aged 12 years in Fujian province has no statistical significance in different gender or between city and countryside.But the caries prevalence of female was higher than that of male when used by sharp dental probe.About 23.23% was found in different detection rates when using two dental probes.Compared to the sampling investigation of Fujian province in 2000 year,the caries prevalence has no obvious increase.Conclusion:The sharp probe has higher detection rate than CPI probe.

Effects of platelet-rich plasma (PRP) on the periodontal healing of replanted teeth / 实用口腔医学杂志

Objective:To evaluate the influence of platelet-rich plasma(PRP) on periodontal healing of replanted teeth in 6 Beagle dogs.Methods:The second incisors were pulled out and re-implanted after 60 min dry storage.In group A,PRP were applied before replantation,and 7days later the teeth received root canal treatment.In group B,PRP were applied before replantation either,but without root canal treatment.In group C,PRP were not applied and 7 days later,the teeth received root canal treatment.In group D,no PRP were applied and no root canal treatment.All the dogs were sacrificed after 12 weeks and the histological parameters were observed.Results:There were no significant differences between group A and C.Group B showed a lower incidence of ankylosis,and higher incidence of healed PDL compared with group D.Conclusion:Under the definite condition,PRP can help to decrease the incidence of ankylosis and increase the incidence of healed PDL in groups that the pulps were not removed.

Computer analysis of the color in ShoFu colormeter plate / 实用口腔医学杂志

Objective: To study the color in ShoFu colormeter plate. Methods: The values of color degree of the cervical, body and incisal end of various teeth in the ShoFu colormeter plate were measured by digital camera and analyzed by computer using CIE1976L*a*b* system and JISZ8722 standard. Results: In ShoFu plate the brightness in the cervical, body and incisal end of various teeth changed following a certain rule, the brightness in group R and VR were higher than that in group A. While the values of color degree in other groups changed without any rule. Conclusion: The digital camera and computer may be used in dental color study.