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Objective To investigate the difference and correlation between venous blood of pregnant women and neonatal um-bilical cord blood .Methods A total of 319 pregnant women and their newborns in People′s Hospital of Longhua District from Jan-uary 2013 to August 2016 were selected as the objects in this research ,fasting venous blood samples of all the pregnant women were collected at the labor day ,umbilical cord blood samples of the infants were collected at the end of delivery .Serum alkaline phospha-tase(ALP) ,pseudocholine esterase(PCHE) ,prealbumin(PA) ,retinol binding protein(RBP) ,urea nitrogen(BUN) ,creatinine(CR) , uric acid(UA) ,total cholesterol(TC) ,triglyceride(TG) and other indicators were detected in all the blood samples .Results ALP , PCHE ,PA ,RBP ,TC and TG of venous blood samples from pregnant women were significantly higher than those of newborn um-bilical cord blood samples ,the differences were statistically significant (P<0 .05) ,but there were no significant differences on BUN , CR ,UA(P>0 .05) .There were positive correlations between venous blood from pregnant women and umbilical cord blood samples on serum PCHE ,PA ,RBP ,BUN ,CR ,UA(P<0 .05) ,while there was no correlation on serum ALP ,TC ,TG(P>0 .05) .Conclusion There are different constituent in venous blood from pregnant women and newborn umbilical cord blood ,and indicators in new-born umbilical cord blood such as PCHE could be effected by the pregnant women .
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Objective To investigate the difference and correlation between venous blood of pregnant women and neonatal um-bilical cord blood .Methods A total of 319 pregnant women and their newborns in People′s Hospital of Longhua District from Jan-uary 2013 to August 2016 were selected as the objects in this research ,fasting venous blood samples of all the pregnant women were collected at the labor day ,umbilical cord blood samples of the infants were collected at the end of delivery .Serum alkaline phospha-tase(ALP) ,pseudocholine esterase(PCHE) ,prealbumin(PA) ,retinol binding protein(RBP) ,urea nitrogen(BUN) ,creatinine(CR) , uric acid(UA) ,total cholesterol(TC) ,triglyceride(TG) and other indicators were detected in all the blood samples .Results ALP , PCHE ,PA ,RBP ,TC and TG of venous blood samples from pregnant women were significantly higher than those of newborn um-bilical cord blood samples ,the differences were statistically significant (P<0 .05) ,but there were no significant differences on BUN , CR ,UA(P>0 .05) .There were positive correlations between venous blood from pregnant women and umbilical cord blood samples on serum PCHE ,PA ,RBP ,BUN ,CR ,UA(P<0 .05) ,while there was no correlation on serum ALP ,TC ,TG(P>0 .05) .Conclusion There are different constituent in venous blood from pregnant women and newborn umbilical cord blood ,and indicators in new-born umbilical cord blood such as PCHE could be effected by the pregnant women .
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Objective To explore risk factors for healthcare-associated infection (HAI)in patients undergoing craniocerebral operation,and provide guidance for the prevention of HAI in patients in department of neurosurgery. Methods 200 patients who underwent craniocerebral operation in a department of neurosurgery from November 2013 to November 2014 were surveyed,risk factors for HAI were analyzed.Results Among 200 patients undergo-ing craniocerebral operation,81 patients developed 99 cases of HAI,HAI rate was 40.50%,HAI case rate was 49.50%;the top five HAI sites were lower respiratory tract,urinary tract,intracranial site,bloodstream,and in-testinal tract.Univariate analysis showed that patients’age ≥60,Glasgow Coma Scale (GCS)<15,intraoperative blood loss ≥800 mL,staying in intensive care unit(ICU),indwelling gastric tube,ventricular drainage,using ventilator,tracheotomy,and using H2 receptor antagonists were important risk factors for HAI in patients undergo-ing craniocerebral operation (all P <0.05).Multivariate logistic regression analysis showed that patients’age ≥60, GCS<15,staying in ICU,and using H2 receptor antagonists were independent risk factors for HAI in patients un-dergoing craniocerebral operation.Conclusion Strengthening the surveillance of HAI patients undergoing cranioce-rebral operation and realizing risk factors for HAI are helpful for taking comprehensive prevention measures and re-ducing the incidence of HAI.
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Objective To investigate the distribution and antimicrobial resistance profile of clinical gram-negative bacterial isolates in the First Afifliated Hospital of Nanjing Medical University during 2014.Methods Bacteria identiifcation was performed by API system or the VITEK-2 Compact automatic identiifcation system. Disk diffusion susceptibility testing or VITEK-2 Compact automatic identification system was used to determine the susceptibility to antimicrobial agents. All data were analyzed using WHONET 5.6 software.Results Among the total 7 931 clinical isolates in 2014, gram-negative bacteria accounted for 64.2% (5 088/7 931). The top three pathogens wereE. coli,A. baumannii andK. pneumoniae. Notably, during the year 2014, 195 strains of carbapenem-resistantEnterobacteriaceaewere isolated, about 6.9% of all theEnterobacteriaceae isolates. Meanwhile, 613 (66.5%) strains of multiple drug resistantA. baumannii and 197 (28.7%) strains of multiple drug resistantP. aeruginosa were isolated.Conclusion During the year 2014, the resistance of the gram-negative bacteria in this hospital is mainly characterized by carbapenem-resistantEnterobacteriaceae, multiple drug resistant A. baumanniiand multiple drug resistantP. aeruginosa. Surveillance of antimicrobial resistance is beneifcial for rational use of antibiotics.
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@#Two simple and efficient methods have been developed for screening and identification of natural peroxynitrite scavengers in Radix Scrophulariae (RS). Method I was based on HPLC-DAD-(luminol-peroxynitrite)-CL techniques combined with Q-TOF MS/MS analysis, while method II was based on the pre-column reaction with peroxynitrite followed by HPLC separation with Q-TOF MS/MS analysis. Five active constituents, P1(decaffeoylacteoside), P9(eoside), I6(6″-O-feruloylharpagide), P11(cis-acteoside)and P13(angoroside), were found to possess potential peroxynitrite-scavenging activity by method I, while P9 and P13 were also screened by method II. Method I requires more complex apparatus, but has advantages on simple detection and high sensitivity. Method II requires simpler apparatus than method I, but with more tedious detection and lower sensitivity. However, the methods established above would provide new ways for rapid detection of natural peroxynitrite-scavenging compounds in RX complex matrices.
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Fanconi's anemia (FA) is an autosomal recessive disease featuring a great diversity of clinical symptoms, including congenital malformation, growth retardation and bone marrow failure. Cells obtained from FA patients show a specific hypersensitivity to crosslinking agents such as mitomycin C (MMC). In this study, MMC-induced chromosome breakage tests have been done on 27 healthy controls and 51 patients with bone marrow failure [including 48 patients with aplastic anemia (AA) and 3 patients with FA before cytogenetic analysis]. The results showed that: (1) Diagnosis of 4 FA cases was confirmed, and one of them was the correction of clinical misdiagnosis; bone marrow failure combined with congenital malformation was observed in a few of non-FA aplastic anemia patients, while 1 FA patient lacked congenital abnormality and underdiagnosed before cytogenetic analysis. The data confirmed that misdiagnosis or underdiagnosis of FA could be caused without cytogenetic study. (2) Spontaneous chromosome breakages observed in FA patients were the same as those in AA patients and healthy controls. MMC-induced chromosome breakages observed in FA patient cells were much higher than those in AA patients and healthy controls, especially, metaphases containing more than 5 breakages were easily found in FA lymphocytes treated with 50 ng MMC. (3) Mosaic was found in one of the 4 FA patients. MMC-induced chromosome breakage test at different MMC concentrations could help to dignosis of FA mosaic patient.