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Clinical and Experimental Vaccine Research ; : 82-86, 2018.
Artículo en Inglés | WPRIM | ID: wpr-739633

RESUMEN

To develop the large scale serological assay for severe fever with thrombocytopenia syndrome virus (SFTSV) infection, we evaluated two different enzyme-linked immunosorbent assay (ELISA) methods using nucleocapsid protein (NP) and Gn proteins of CB1 (genotype B) SFTSV strains. The NP-based ELISA tests showed more sensitive with broad cross-reactivity between two different genotype A and B strains compared with those of Gn-based ELISA tests. However, Gn-based ELISA showed more genotype specificity and specificity. These result suggested that NP-based ELISA test could be applicable for general sero-prevalence studies of SFTSV infections, while Gn-based ELISA could be applicable for a certain specific genotype sero-prevalence study.


Asunto(s)
Diagnóstico , Ensayo de Inmunoadsorción Enzimática , Fiebre , Genotipo , Proteínas de la Nucleocápside , Sensibilidad y Especificidad , Trombocitopenia
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