Improved liquid chromatography combined with pulsed electrochemical detection for the analysis of etimicin sulfate / 中国药学杂志

OBJECTIVE: To establish an improved LG method combined with pulsed electrochemical detection for the analysis of etimicin sulfate. METHODS: The mobile phase was composed of 40 mL of acetonitrile and 960 mL of an aqueous solution containing 15 mL · L-1 of trifluoroacetic acid, 500 μL · L-1 of pentafluoropropionic acid, 8 mL · L-1 of sodium hydroxide (50%) and 1.5 g · L-1 of sodium sulfate. The pH of the aqueous solution was adjusted to 3.5 with 50% NaOH solution. A pulsed electrochemical detector, which was kept at 35 C in a hot air oven was adopted. The electrochemical cell consisted of a working electrode, a pH-Ag/AgCl reference electrode and a titanium counter electrode. The working electrode was a gold electrode (diameter 3 mm) and a quadruple-potential waveform (QPW) was selected as detection waveform. The 0.8 mol · L-1 NaOH solution was added post column at a flow rate of 0.4 mL · min-1. RESULTS In total, 22 impurities could be separated. The LOD and LOQ of etimicin were found to be 2 ng and 6 ng respectively. The linearity of the calibration curve for etimicin ranged from 0.24 to 45 μg · mL-1 with a coefficient of correlation equal to 0.9997. The repeatability RSDs (n=6) of the content and total impurities in one sample were 0.7% and 1.72% respectively. The inter-day repeatability RSDs (n=18) of the content and total impurities in one sample were 0.98% and 1.71% respectively. The sample solution was stable within 12 h. CONCLUSION: Compared with previously published methods, this improved method shows higher sensitivity, better separation ability and robustness and has been incorporated by the Chinese Pharmacopoeia (Ch. P.) 2015 for analysis of etimicin sulfate.

Clinical observation on treatment of non-gonococcal cervicitis by integrative medicine / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To explore the integrative medicinal therapy for non-gonococcal cervicitis (NGC) in order to elevate the therapeutic effect for patients treated in vain after long-term application of antibiotics.</p><p><b>METHODS</b>Fifty patients with NGC were treated with Qingyuan decoction combined with antibiotics, and other 46 patients were treated with antibiotics alone for control.</p><p><b>RESULTS</b>The cure rate and significant effective rate was 90.0% (45/50) and 65.2% (30/46) in the treated group and the control group, it was significantly different between the two groups (chi2 = 9.58, P < 0.01). The disappearance rate of symptom was 88.1% and 41.4% in the two group after treated for two weeks respectively, and it was also significantly different (chi2 = 12.42, P < 0.01).</p><p><b>CONCLUSIONS</b>The therapeutic effect of NGC treated by sensitive antibiotics combined with Qingyuan decoction is better than that treated with western medicine only.</p>

Clinical study of yishen jianpi yangxue tongli therapy in treating polycystic ovary syndrome / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To observe the effect of Yishen Jianpi Yangxue Tongli Therapy (YJYTT) in treating polycystic ovary syndrome (PCOS) and to explore the therapeutic mechanism of YJYTT.</p><p><b>METHODS</b>One hundred and seven PCOS patients with syndrome differentiation of TCM as Pi-Shen Yang deficiency were randomly divided into two groups, the treated group (n = 76) was treated with Chinese herbal medicine, while the control group (n = 31) was treated by clomiphen. The therapeutic effect in both groups and the change of body mass index (BMI), Ferriman-Gallway (F-G) score, serum sex hormones and serum oral glucose tolerance test (OGTT) before and after treatment were observed.</p><p><b>RESULTS</b>The comparison of total effective rate between two groups was insignificantly different, but the pregnancy rate of the treated group (65.7%) was markedly higher than that of control group (25.0%, P < 0.01). Also, significant difference was shown in the aspect of BMI, F-G score, serum testosterone (T), luteinizing hormone (LH) and OGTT, etc. between the two groups (P < 0.05).</p><p><b>CONCLUSION</b>The YJYTT could markedly improve the clinical symptoms and pregnancy rate of PCOS patients, particularly showing good effect to the clomiphen resistant patients. The mechanism possibly was due to its regulation on hypothalamus-pituitary-ovarium axis and reducing the insulin resistance.</p>