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Acta Physiologica Sinica ; (6): 417-421, 2003.
Artículo en Chino | WPRIM | ID: wpr-290950

RESUMEN

To investigate the changes in the nitric oxide (NO) contents, nitric oxide synthase (NOS) activity and inducible nitric oxide (iNOS) mRNA expression in arginine vasopressin (AVP)-induced cardiac fibroblasts (CFs) in vitro and its relation to nuclear factor-kappaB (NF-kappaB), CFs were isolated by trypsin digestion method. Nitric acid reductase method, spectrophotometry, reverse transcription-polymerase chain reaction (RT-PCR), immunofluorescence-interactive laser cytometer techniques and Western blotting were used respectively to detect NO contents, NOS activity, iNOS mRNA expression and the activation of NF-kappaB in CFs. AVP increased NO contents, NOS activity and iNOS mRNA expressions in a concentration-dependent manner; NF-kappaB was activated and mobilized from cytoplasm to nucleus in AVP-induced CFs; PDTC, one of the inhibitors of NF-kappaB, could inhibit aforementioned increments. It is suggested that the increases in NO contents, elevation of NOS activity and increment of iNOS mRNA expression may be mediated through NF-kappaB activation pathway in cultured CFs induced by AVP, and that NF-kappaB is involved in the occurrence and development of myocardial fibrosis.


Asunto(s)
Animales , Ratas , Animales Recién Nacidos , Arginina Vasopresina , Farmacología , Células Cultivadas , Fibroblastos , Biología Celular , Metabolismo , Miocitos Cardíacos , Biología Celular , Metabolismo , FN-kappa B , Metabolismo , Óxido Nítrico , Metabolismo , Óxido Nítrico Sintasa de Tipo II , Genética , Metabolismo , ARN Mensajero , Genética , Metabolismo , Ratas Sprague-Dawley
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