Analysis of monosaccharide composition and monosaccharide ratio in propylene glycol alginate sodium sulphate drugs by high performance anion exchange chromatograghy -pulsed amperometric detection / 药学学报

The paper aims to establish the method to determine the monosaccharide composition and monosaccharide ratio in propylene glycol alginate sodium sulphate (PSS). Samples were hydrolyzed with trifluoroacetic acid, neutralized with sodium hydroxide solution after the reaction conditions for sample pretreatment were optimized via orthogonal analysis. A high performance anion exchange chromatograghy (HPAEC) coupled with pulsed amperometric detector (PAD) was performed on a CarboPac®PA20, using 200 mmol·L-1 sodium hydroxide solution and 1 mol·L-1 sodium acetate solution as mobile phase. The established HPAEC-PAD method was validated by testing the linear relationship, precision and accuracy, and showed exclusive, sensitive, rapid and wide use. The monosaccharide composition of PSS from different manufacture can be accurately determined with great significance for the structural identification of PSS.

The second international collaborative study on molecular weight of heparin sodium reference standard / 中国药学杂志

OBJECTIVE: To apply the heparin sodium molecular weight reference standard calibrated in the first study period to GPC method and assesse the GPC method.

The first international collaborative study on molecular weight of heparin sodium reference standard / 中国药学杂志

OBJECTIVE: To calibrate the molecular weight of reference standard of heparin sodium.