Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
1.
Chinese Journal of Oncology ; (12): 134-136, 2003.
Artículo en Chino | WPRIM | ID: wpr-347477

RESUMEN

<p><b>OBJECTIVE</b>To establish human multidrug-resistant lung carcinoma cell line (D6/MVP) with its characteristics studied.</p><p><b>METHODS</b>Intermittent administration of high-dose MMC, VDS and DDP (MVP) was used to induce human lung carcinoma cell line (D6) to a multidrug-resistant variety (D6/MVP). MTT assay was used to study the multidrug resistance of D6/MVP to multianticarcinogen. Flow cytometry was used to study the cell cycle distribution and the expression of P-gp, multidrug resistance-associated protein (MRP) and GSH/GST.</p><p><b>RESULTS</b>1. D6/MVP was resistant to many anti-tumor agents, with the IC(50) 13.3 times higher and the drug resistance 2 - 6 times higher than D6, 2. The multiplication time of D6/MVP was prolonged and the cell number of S-phase decreased while that of G1- and G(2)-phase increased and 3. The expression of P-gp and MRP was enhanced significantly (96.2% vs 51.7%), but the expression of GSH/GST kept stable.</p><p><b>CONCLUSION</b>D6/MVP is a multidrug-resistant cell line possessing the basic characteristics of drug-resistance.</p>


Asunto(s)
Humanos , Miembro 1 de la Subfamilia B de Casetes de Unión a ATP , Protocolos de Quimioterapia Combinada Antineoplásica , Usos Terapéuticos , Línea Celular Tumoral , Cisplatino , Resistencia a Múltiples Medicamentos , Citometría de Flujo , Glutatión , Glutatión Transferasa , Metabolismo , Neoplasias Pulmonares , Quimioterapia , Patología , Mitomicinas , Vinblastina
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA