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1.
Journal of Southern Medical University ; (12): 1127-1131, 2007.
Artículo en Chino | WPRIM | ID: wpr-337314

RESUMEN

To investigate the interaction between tumor necrosis factor alpha (TNF alpha) mimotopes and TNF alpha-binding peptides screened from random phage display peptide library with TNF alpha mimotopes displayed on phage clone as the target, the computational docking program AutoDock (with confirmation calculations using Discover) was used to predict and analyze the binding modes of LLT-18 (TNF alpha binding peptide, sequence EHMALTYPFRPP) with TNF alpha, after which LCS-7 (TNF alpha mimic phage clone, displayed positive sequence c-RRPAQSG-c) was docked to LLT-18 manually. The binding between LLT-18 and TNF alpha or LCS-7 was stabilized predominantly through electrostatic interaction and H-bond formation. The Arg residues in TNF alpha or LCS-7 were important for their interaction with LLT-18. For LLT-18, the key amino acid residues were Glu1, His2, Met3 and Tyr7. These results suggest the feasibility of screening ligand to single epitope with specific phage clone as the target, and of predicting the interaction between small peptides by computer-aided molecular modeling.


Asunto(s)
Animales , Humanos , Secuencia de Aminoácidos , Anticuerpos Monoclonales , Alergia e Inmunología , Biotinilación , Simulación por Computador , Evaluación Preclínica de Medicamentos , Métodos , Epítopos , Alergia e Inmunología , Ligandos , Modelos Moleculares , Oligopéptidos , Química , Metabolismo , Biblioteca de Péptidos , Conformación Proteica , Solubilidad , Factor de Necrosis Tumoral alfa , Química , Alergia e Inmunología , Metabolismo
2.
Journal of Experimental Hematology ; (6): 263-267, 2001.
Artículo en Chino | WPRIM | ID: wpr-258020

RESUMEN

The structure analysis of porcine hemoglobin alphabeta dimer and the calculation of solvent accessible surface of the amino acids showed the epsilon-amino groups of the lysine are suitable for modification by polyethylene glycol (PEG). The modification of the lysine residues will not affect the carring oxygen capacity of Hb. Three types of linker have been designed to connect PEG and porcine hemoglobin. The lysines between porcine and bovine hemoglobin (pHb and bHb) are highly conserved, but the solvent accessible surface of conserved lysines are different. These suggested that the properties of homologous proteins are similar in pHb and bHb, but the characteristic derived from the homology analysis will be deviated from the actual status. The results of molecular dynamics simulation suggested that the chemical modified porcine hemoglobin would be no immunogenicity.

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