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@# ObjectiveTo explore the possibility of repairing sciatic nerve gap of rats with artifical nerve graft.MethodsA novel artifical nerve guide was developed and used to suture the 15 milimeter long right sciatic nerve gap of 10 rats, other 7 rats were the control with the right sciatic nerve gap alone.2 and 4 monthes after operation, immunohistochemistry, Osmium staining, Bodian staining,motor end plate staining,WGA-HRP stain tracing have been done to observe the effect of repairing.Results2 months after operations, the sciatic nerve gap were repaired by the regeneration nerve.There was not evident inflammation in the defects.ConclusionsThe artifical nerve graft can induce the nerve to regenerate.
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@#ObjectiveTo study the effect the chitosan tube combined with the bio active carrier system on inducing nerve axon regeneration of rats with spinal cord injury.Methods50 female Wister rats were characterized by the right spinal cord hemisections at the seventh and eighth thoracic segment to make the model of spinal cord hemisection. The chitosan tube serving as a regenerative loculus was implanted in the defect location of the experimental models.In the experimental group,the bio active carrier system was injected into the chitosan tube,while in control group injected nothing.The drua was sutured to restore cerebrospinal fluid circulation.Results6 months and 12 months after operation,the regenerative nerve axon had passed the defect area of spinal cord in the experiment group. According to WGA-HRP anterograde axonal tracing study, some TMB-positive axons were observed in the distal graft host interface,and came into the host environment. TEM-ultrastructure indicate some neonate synapse, myelinated nerve fibers.In the control group,few regenerative axon could be seen, there was no regenerative axon pass the middle of the tube.ConclusionsThe chitosan tube in which the bio active carrier system was injected can induce the spinal cord nerve axon regeneration.
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A comparative study on the cellular survival of grafts and the behavior improvement in the rats was performed following tissue transplantation of either fresh or freezing-stored adrenal medulla. The fresh or freezing-stored adrenal medullary tissues were transplanted into the head of caudate nucleus in the animal models with unilateral 6-hydroxydopamine lesions of the substantia nigra. Experimental groups got some improvement after operation in rotation behavior induced by apomorphine, and the differences were significant between experimental and control groups. Among the transplanted rats, those received fresh tissue seemed to show more improvement than those received freezing-stored tissue, but the statistical difference was not significant. With regard to fluorescent intensity of the adrenal cells, it showed to be stronger in the experimental group received fresh graft than the group received freezing-stored graft, but the difference was no statistically significant either. No essential difference could be found between experimental groups as to the cellular apperance and staining features in the grafts.
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Fourteen adult and immature rabbits were used for the study of laminar scheme of the gray matter in the spinal cord. The spinal sections were cut transversally or sagitally into 80 ?m, 60 ?m, 15?m, and 2?m-thick sections. The sections were stained with toluidine blue or crecyl fast violet for cell body and with Luxol fast blue for myelin sheath. 2?m-thick sections were only stained with pphenylenediamine. In addition, the spinal sections from 2 cases of the animals were treated histochemically for demonstrating AChE activity. According to the Rexed's principle lamination for the cat, we have found that the cytoarchitectonic organization of the rabbit spinal cord was found to be basically similar to that of the cat except for some differences about the extension and structures of the laminae.