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Journal of Southern Medical University ; (12): 525-531, 2016.
Artículo en Chino | WPRIM | ID: wpr-264010

RESUMEN

<p><b>OBJECTIVE</b>To investigate the ability of Porphyromonas gingivalis to invade human periodontal ligament cells (hPDLCs) and the effect of intracellular P. gingivalis on cell proliferation and osteogenic differentiation in vitro.</p><p><b>METHODS</b>The invasion ability of P. gingivalis in hPDLCs was tested using an antibiotic protection assay at the multiplicity of infection (MOI) of 10 and 100. The proliferation of the infected cells was detected using a CFDA-SE kit, and the cells were sorted by fluorescence-activated cell sorting (FACS) followed by alizarin red staining for detecting mineralization nodules deposition; real-time PCR was used to examine the expression of Runx2 mRNA in the cells.</p><p><b>RESULTS</b>P. gingivalis actively invaded hPDLCs, and the internalized P. gingivalis was able to resist antibiotic treatment. The cells infected by P. gingivalis exhibited no significant suppression of cell proliferation, but showed significantly lowered capacity for osteogenic differentiation, down-regulated RUNX2 mRNA expression, and reduced mineral deposition.</p><p><b>CONCLUSION</b>Intracellular P. gingivalis does not significantly affect the proliferation of hPDLCs but inhibits osteogenic differentiation of the cells.</p>


Asunto(s)
Humanos , Diferenciación Celular , Proliferación Celular , Células Cultivadas , Subunidad alfa 1 del Factor de Unión al Sitio Principal , Metabolismo , Citometría de Flujo , Fluoresceínas , Osteogénesis , Ligamento Periodontal , Biología Celular , Microbiología , Porphyromonas gingivalis , ARN Mensajero , Reacción en Cadena en Tiempo Real de la Polimerasa , Succinimidas
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