RESUMEN
Linaoxin, a herhal preparatlon composed of Salvia miiltiorrhiza, Ligusticum wallichii,Pueraria lobata,and Polygonum multiflorum etc., at doses of O. 25 and o. 5g/kg (ig)significantly improved the neural symptoms of experimental cerehral infarction and reduced the size of infarcted area in rats. At the same doses it also significantly prolonged the hreathing time of mice after decapitation and showed effects on brain energyrnetabolism including the increase of brain ATP and phosphocreatine (Pcr)leveIs and decrease of brain lacticacid (LA)level. Linaoxin suspension at concentrations of l:
RESUMEN
A simple, rapid and sensitive method for reversed phase HPLC determination of N-acetyl-L-tryptophan (N-ATRP) and its metabolite L-tryptophan (TRP) in rabbit serum was developed based on their intrinsic fluorescence using excitation and emission wavelengths of 280 and 360 nm, respectively. The separation of two amino acids was achieved on an ODS C18 column with a mobile phase of 0. 1 mol ?L-1 NaAc-25% methanol (pH 5. 7) at a flow rate of 0. 5 ml ?min-1. The calibration curves for N-A-TRP and TRP provided the linear range about1.0 - 17. 0mg ?L-1(r=0. 9962 and 0.9978, respectively). The recoveries greater than 98% and good reproducibility were achieved with rabbit serum. The concentration-time curve of N-ATRP after iv administration was determined in rabbits. Results showed that N-ATRP was quickly converted to TRP with a half-life of 8. 5 min. The decrese of N-ATRP accorded well with the rise of TRP.