Study on Relationship Between FABP Family and Risk and Prognosis of Gastric Cancer and its Function / 胃肠病学

Background: Gastric cancer is a common gastrointestinal tumor. Fatty acids play a vital role in the development of gastric cancer. The function of fatty acids requires the participation of fatty acid-binding proteins (FABPs). Aims: To systematically analyze the influence of FABPs family on risk and prognosis of gastric cancer and understand the potential role of FABPs family. Methods: The ONCOMINE database was used to analyze the differential expression of FABPs family member mRNA between gastric cancer and adjacent normal tissue, and the GEPIA database was further used for verification. The relationship between FABPs family member and survival of gastric cancer patients was analyzed. Then The Human Protein Atlas database was used to further analyze the differential expression of FABP family protein in gastric cancer. STRING and DAVID databases were used for protein-protein interaction (PPI) analysis and GO, KEGG enrichment analysis. Results: Compared with normal gastric tissue, the expressions of FABP3/4/7 in gastric cancer were significantly down-regulated, while FABP1 expression remained controversial. Survival analysis showed that patients with low expressions of FABP3/4/8 mRNA had a higher overall survival rate. GO analysis showed that FABPs family mainly enriched in biological process, cytoplasm, molecular function change. KEGG enrichment analysis showed that FABPs were mainly involved in PPAR signaling pathway, fat digestion and absorption signaling pathway. Conclusions: FABP3/4/7 can be used as risk markers and FABP3/4/8 as prognostic markers for gastric cancer. The effect of FABPs may be related to the PPAR pathway and lipid metabolism pathway.

Comparison of metabolite extraction from Boea hygrometrica by gas chromatography/mass spectrometry / 生物工程学报

This study is aiming to establish an efficient metabolite extraction method for exploration of molecular mechanisms of desiccation tolerance of the resurrection plant Boea hygrometrica using a metabolomics approach. The extracts of metabolite in B. hygrometrica using methanol solution (method A) and methanol-chloroform-water solution (method B) were analyzed by gas chromatography-mass spectrometry (GC-MS). The total numbers of chromatographic peaks, extraction efficiency, retention time and the peak stability were compared. The results showed that for fresh materials, the total chromatographic peak number of method B is more than that of method A; the extraction efficiency of nine representative metabolites by method B is higher than that by method A; the comparison of 10 random chromatographic peaks revealed that the relative standard deviation (RSD) values of the retention time are less than 1% for both methods, whereas the RSD values of the extraction efficiency is different. The percentage of peaks that owned RSD values of the extraction efficiency higher than 10% is 50% for method A and 100% for method B. In addition, method B was also efficient for dry materials from B. hygrometrica. The number of chromatographic peaks, RSD value of retention time and extraction efficiency of dry materials was similar to that of fresh materials using method B, but decreased sharply using method A. Putting together, our study provided evidence that method B is an efficient extraction method for further analysis of metabolites from this resurrection species.

The study on changes of serum gastrin level and its influencing factors in carcinogenesis of gastric mucosa / 中国实用内科杂志

Objective To explore the common factors' influence on serum gastrin level,and reveal the value of serum gastrin in the diagnosis of gastric precancerous lesions and gastric cancers.Methods Serum gastrin level of 3 906 serum samples was detected by ELISA.Results Serum gastrin level of patients over 60 years old was distinctively higher than that of other age groups.Serum gastrin level in antral diseases was higher than that in diseases of the body.HP positive group was higher than the negative one.The level progressingly increased from normal group to superficial gastritis and to gastric erosion or ulcer;and it decreased from gastric erosion or ulcer to atrophic gastritis,while it increased from atrophic gastritis to dysplasia,and it decreased from dysplasia to gastric cancer.Serum gastrin level was distinctively lower in gastric cancer than in other groups.Conclusion For people over 60 years old,their serum gastrin level has the tendence to increase.The location of gastric disease is an important factor affecting serum gastrin level.HP can increase the serum gastrin level.During gastric precancerous stage,the serum gastrin level may gradually increase with the diseases' progression,and decrease when gastric cancer is formed.Serum gastrin is a good marker to differentiate benign and malignant gastric diseases.

Changes in serum osteopontin level and its clinical significance in carcinogenesis and development of gastric cancer / 中华检验医学杂志

Objective To reveal the change tendency of serum osteopontin(OPN) in carcinogenesis and development of gastric cancer(GC), and explore the usefulness of serum OPN in the diagnosis of GC and the differential diagnosis of early GC and advanced GC.Method Serum OPN levels were detected by enzyme-linked immunosorbent assay, including 70 advanced GC, 24 early GC, 47 dysplasia, 42 intestinal metaplasia, 46 chronic superficial gastritis patients and 38 normal controls.Results Serum OPN level in GC group (57.03 ng/ml) was significantly higher than those in other groups.Serum OPN level in advanced GC group (66.05 ng/ml) was significantly higher than that in early GC group (41.93 ng/ml), serum OPN level in early GC group was significantly higher than those in dysplasia, intestinal metaplasia and chronic superficial gastritis, and serum OPN level in the latter three groups was significantly lower than that in normal controls (P