RESUMEN
Objective To analyze the expression of insulin-like growth factor-1(IGF-1)in the serum of patients with pituitary adenomas and compare them according to different standards,investigating the expression of IGF-1 in pituitary adenoma and its clinical significance.Methods A total of 156 cases of pituitary adenomas admitted to the Second Affiliated Hospital of Kunming Medical Univer-sity from January 2019 to June 2021 were selected as the experimental group,and a total of 22healthy subjects during the same period were selected as the control group.To determinate the IGF-1 level by magnetic particle chemical luminescence immunoassay.The tumor di-ameter was obtained by combining the diameter measured on magnetic resonance imaging(MRI)with the diameter of postoperative tumor pathological specimen.The experimental group were divided into groups according to different clinical characteristics,and to compare the differences in IGF-1 levels between each subgroup and the control group.Results The levels of IGF-1 in patients with somatotroph adenoma,dual hormone and multihormone adenoma were higher than those in the control group and other types,and the differences were statistically significant(P＜0.05);the level of IGF-1 in giant adenoma patients was higher than those in the control group and other types,and the difference was statistically significant(P＜0.05);the level of IGF-1 in patients with invasive pituitary adenoma was higher than that in non-invasive patients and controls,and the difference was statistically significant(P＜0.05);Logistic regression a-nalysis showed that IGF-1 level and tumor diameter were risk factors for invasiveness of pituitary adenomas,the higher the IGF-1 level and the larger the tumor diameter,the higher the invasiveness risk;the level of IGF-1 in patients with pituitary adenoma within one month after surgery was significantly lower than that before surgery,and the difference was statistically significant(P＜0.05).Conclu-sion IGF-1 has a certain value in the diagnosis of different functional types of pituitary adenomas and the early invasiveness judgment of pituitary adenomas,and is expected to be used as a reliable indicator for postoperative follow-up.
RESUMEN
<p><b>OBJECTIVE</b>To observe the effects of electroacupuncture (EA) at acupoints with different nerve segments on gastric emptying in oxytocin (OT) gene knockout mice, and to explore the role of paraventricular nucleus OT in EA regulating gastric function.</p><p><b>METHODS</b>Twenty OT knockout mice and twenty wild-type mice (8-9 weeks old, male and female in half) were divided into a control group, a Zusanli (ST 36) group, a Neiguan (PC 6) group and a Weishu (BL 21) group randomly, 5 mice in each group. The mice in the control group received no acupuncture; the mice in the Zusanli (ST 36) group, Neiguan (PC 6) group and Weishu (BL 21) group were treated with EA at unilateral "Zusanli" (ST 36), "Neiguan" (PC 6) and "Weishu" (BL 21) for 15 min respectively. Subsequently, the mice were treated with intragastric administration with milk loaded with technetium-99m diethylene triamine pentaacetic acid (Tc-DTPA), immediately followed by scanning of single photon emission computed tomography (SPECT) to test gastric emptying for 50 min; the original data was collected and analyzed to obtain the half gastric emptying time (GET 1/2), 50-min stomach residue rate and gastric emptying curve.</p><p><b>RESULTS</b>The wild-type mice group:compare with the control group, after EA at "Zusanli" (ST 36) and "Neiguan" (PC 6), the GET 1/2 and 50-min stomach residue rate were significantly reduced, which could improve the gastric emptying of wild-type mice (<0.01,<0.05); after EA at "Weishu" (BL 21), the GET 1/2 and 50-min stomach residue rate were significantly increased, which could inhibit the gastric emptying of wild-type mice (<0.01,<0.05). The OT gene knockout mice group:compared with the control group, EA at "Zusanli" (ST 36) and "Neiguan" (PC 6) had no significant effects on gastric emptying (both>0.05); after EA at "Weishu" (BL 21), the GET 1/2 and 50-min stomach residue rate were significantly increased, which could inhibit the gastric emptying of OT gene knockout mice (both<0.01). Gastric emptying curve:the gastric emptying of OT gene knockout mice was significantly faster than that of wild-type mice.</p><p><b>CONCLUSIONS</b>The effects of EA at acupoints with different nerve segments on gastric emptying in mice are different, and the knockout of OT gene could accelerate the gastric emptying, and influence the acupuncture effect of several acupoints. It is suggested the paraventricular nucleus OT neurons, as the initiating device of autonomic nervous pathways, might participate in EA regulating the gastric function to inhibit the gastric emptying, with the form of neural and neuroendocrine.</p>
RESUMEN
<p><b>OBJECTIVE</b>To investigate the antimicrobial resistance status and pulsed field gel electrophoresis (PFGE) patterns of Salmonella Enteritidis (S.Enteritidis) strains in Henan province.</p><p><b>METHODS</b>S. Enteritidis strains were isolated from seven sentinel hospitals from March 2011 to December 2013. According to molecular typing and Salmonella (Kirby-Bauer, K-B) drug susceptibility testing method published by the international PulseNet bacterial infectious disease monitoring network and USA Clinical and Laboratory Standards Institute (CLSI), we analyzed drug sensitivity of 8 kinds antibiotics and PFGE molecule characteristics of 120 S. Enteritidis isolates from seven sentinel hospitals.</p><p><b>RESULTS</b>Among 120 strains of S. Enteritidis, 77 were isolated from male patients, 43 from female patients. A total of 78 strains S. Enteritidis were isolated from young children ranged from 0 to 5 years old (65.0%), including 57 strains isolated from 6 months to 2 years old (47.5%). The isolated time mainly centralized on May to October of the year, 11 strains isolated in March-April (9.2%), 48 were in May-July (40.0%),54 in August-October (45.0%), 7 in other months, with a typical summer seasonal characteristics. The resistance rate of 120 strains S. Enteritidis to ampicillin was 50.0% (n=60); to ceftazidime was 14.2% (n=17), to cefotaxime was 18.3% (n=22); to cefepime was 5.8% (n=7); to nalidixic acid was 61.7% (n=74); to ciprofloxacin was 8.3% (n=10), to norfloxacin was 5.8% (n=7); to gentamicin was 42.5% (n=51); to streptomycin was 21.7% (n=26); to chloramphenicol was 30.0% (n=36); resistance to methicillin benzyl ammonium was 11.7% (n=14), compound sulfamethoxazole resistance rate was 71.7% (n=86); the tetracycline resistant rate was 47.5% (n=57). All 120 strains of S. Enteritidis had different levels of resistance to 8 kinds of antibiotics, all strains were multidrug resistant strains, 28 isolates were resistant to 3-4 kinds of antibiotics (23.3%), 38 isolates were resistant to 5-6 kinds of antibiotics (31.7%), 39 isolates were resistant to 7-8 kinds of antibiotics (32.5%). All 120 strains of S. Enteritidis were divided into 44 molecular patterns by digestion with XbaI and pulsed field gel electrophoresis. each pattern contained 1-35 strains with similarity ranged from 54.3%-100%. EN14 and EN19 were the main PFGE types, including 35 and 29 strains respectively.</p><p><b>CONCLUSION</b>The status of drug resistance of clinical isolates of Salmonella in Henan province was rather serious, PFGE patterns showed advantages and partial strain's corresponding resistant spectrum have certain relevance and the same aggregation relationship.</p>