RESUMEN
Plant virus is one of the most economical devastating microorganisms for global agriculture. Although several strategies are useful for controlling viral infection, such as resistant breeds cultivation, chemical bactericides treatment, blocking the infection source, tissue detoxification and field sanitation, viral disease is still a problem in agricultural production. Genetic engineering approach offers various options for introducing virus resistance into crop plants. This paper reviews the current strategies of developing virus resistant transgenic plants.
Asunto(s)
Agricultura , Productos Agrícolas , Genética , Virología , Ingeniería Genética , Enfermedades de las Plantas , Virología , Virus de Plantas , Plantas Modificadas Genéticamente , VirologíaRESUMEN
Autophagy is a conserved pathway for the bulk degradation of cytoplasmic components in all eukaryotes. This process plays a critical role in the adaptation of plants to drastic changing environmental stresses such as starvation, oxidative stress, drought, salt, and pathogen invasion. This paper summarizes the current knowledge about the mechanism and roles of plant autophagy in various plant stress responses.
Asunto(s)
Adaptación Fisiológica , Arabidopsis , Genética , Fisiología , Proteínas de Arabidopsis , Genética , Metabolismo , Autofagia , Genética , Resistencia a la Enfermedad , Enfermedades de las Plantas , Alergia e Inmunología , Saccharomyces cerevisiae , Genética , Homología de Secuencia , Estrés FisiológicoRESUMEN
Total DNA of B. amyloliquefaciens and S. aureus were isolated and cut partially with Hind Ⅲ Separately, The genes for barnase and for staphylococcal nucl ease were obtained by PCR and cloned into pGEM7Z-f(+), The sequencing show that the n ucleotide (nt) sequence of the barnase is 99.3% homologous with the previously determimed sequence and the deduced animo acid (an)sequence found to correspond precisely to the previously determined sequence; the nucleotide (nt) sequence of the staphylococcal nuclease is 99.1% homologous with the previously determined sequence and the deduced amino acid (an) sequence 99.3% homologous with the previously determinded sequence.These have built basis of making use of special expression or activation of nuclease to produce the male sterility plant by plant gene engineering and to explore new antiviral strategy.