RESUMEN
BACKGROUND: The chemical characteristics of the exo-secretion from Phellinus linteus (referred to as exo-secretion) including the compositions of amino acids and monosaccharides were investigated. In addition, cytotoxicity of the exo-secretion on 5 tumor cell lines derived from human cancers and its antitumor activity against ascitic sarcoma-180 cells were examined. METHODS: The antitumor activity of exo-secretion from Phellinus linteus was determined by measuring parameters including tumor weight, life span of mice, chemotatic activity of leukocytes, counts of immune cells, and activity of cytokines. RESULTS: The exo-secretion from Phellinus linteus showed no direct cytotoxicity to the five tumor cell lines tested, but it had a strong antitumor activity against sarcoma-180 cells in ICR mice as measured by tumor weight and life span of mice. The exo-secretion stimulated the chemotaxis of leukocytes and production of immune cells and cytokines. CONCLUSION: These results suggest that the exo- secretion from Phellinus linteus do not act as a direct cytotoxic substance to cancer cells but as an immunomodulator.
Asunto(s)
Animales , Humanos , Ratones , Aminoácidos , Línea Celular Tumoral , Quimiotaxis , Citocinas , Leucocitos , Ratones Endogámicos ICR , Monosacáridos , Carga TumoralRESUMEN
To study the relation between ectoparasite, Rickettsia and hantaan virus in bats, four order of Athropoda were collected from Rhinolophus ferrumequinum captured in Cheju and Eptesicus serotinus captured in Chungnamin from July 1989 to March 1998. Also antibody of Rickettsia and hantaan virus were detected by immunofluoroscent antibody technique and RT-PCR. The results are as follows. 1. Five species of Acarina were identified from E. serotinus: Leptotrobidium subakamushi of Trombiculidae, Macronyssus coreanus, Steatonyssuss spinosuss and Steatonychus superans of Macronyssidae, Argas vespertilionis of Metastigmata. 2. Ischnopsyllus needhami of Siphonaptera and Cimex of Hemiptera were identified from E. serotinus. 3. Cyteribia uenoi and Brachytarsina kanoi of Diptera were identified from R. ferrumequinum. 4. The positive rate of rickettsial antibodies in E. serotinus were 17.58%, 15.15%, 22.22%, 52.73% against R. tsutsugamushi, R. typhi, R. sibirica and R. thai tick typhus, respectively. The high positive rate of antibody related to the high content of Arthropoda. 5. The Positive rate of hantaan virus IFA antibodies were 3.4% (27 of 802) and hanntan virus infection rate 36.7% (22 of 60) by RT-PCR in bats. According these result, we showed that certain species of Athropoda isolated play a role as vector of Rickettsia in E. serotinus. Also bats play a role as a reservoir of hantaan virus in nature.
Asunto(s)
Humanos , Anticuerpos , Argas , Artrópodos , Pueblo Asiatico , Chinches , Quirópteros , Dípteros , Virus Hantaan , Hemípteros , Rickettsia , Siphonaptera , Garrapatas , Trombiculidae , Tifus Epidémico Transmitido por PiojosRESUMEN
Hantavirus is a genus of the Bunyaviridae family consisting following serotype groups: Hantaan, Seoul, Puumala, Prospect Hill, Thailand, Belgrade, Thotta palayam, Sin Hombre. Most of Hantavirus group have been associated with many clinically similar disease known collectively as hemorrhagic fever with renal syndrome (HFRS). Hantaan virus is the prototype of the genus hantavirus, originally isolated from Apodemus agrarius. Bat was found as a natural host for Hantaan virus in Lee's lab for the first time. Then, Hantaan-like virus was isolated Hantaan-like virus from bat. To identify hantaviruses that are present in Korea among bats, bats were collected from Jeong-Sun, Won-Joo, Chung-Ju and Hwa-Cheon area, RNA was isolated from lung and serum. RT-PCR was performed with a universal primer from M segment. Nested RT-PCR was carried out to differentiate Hantaan, Seoul and Puumala virus using serotype specific primers. As we expected, Hantaan viruses were detected in bats and Seoul virus was not detected. Interestingly, Puumala viruses were also detected in bats from won-Ju, but not in other areas. Puumala virus is originally isolated from Clethrinomys glareolus, and cause light HFRS. Recently, Paradoxomis webbiana, a wild bird turn out to be a reservoir for Puumala virus in Korea. These data indicate that bat is a new natural reservoir of Puumala virus.
Asunto(s)
Animales , Humanos , Aves , Bunyaviridae , Quirópteros , Virus Hantaan , Orthohantavirus , Fiebre Hemorrágica con Síndrome Renal , Corea (Geográfico) , Pulmón , Murinae , Virus Puumala , ARN , Seúl , Virus Seoul , TailandiaRESUMEN
No abstract available.
Asunto(s)
Animales , Quirópteros , Corea (Geográfico) , Murinae , Reacción en Cadena de la Polimerasa , Virus PuumalaRESUMEN
No abstract available.
Asunto(s)
Quirópteros , Fiebre Hemorrágica con Síndrome Renal , Corea (Geográfico)RESUMEN
No abstract available.
RESUMEN
No abstract available.
Asunto(s)
Aves , Quirópteros , Infecciones por Hantavirus , Orthohantavirus , Corea (Geográfico) , Estudios SeroepidemiológicosRESUMEN
No abstract available.
Asunto(s)
Humanos , Anticuerpos , Pueblo Asiatico , Quirópteros , Virus de la Encefalitis Japonesa (Especie) , Encefalitis Japonesa , Corea (Geográfico)RESUMEN
No abstract available.
RESUMEN
No abstract available.