RESUMEN
On Farm Testing (OFT) in Mungbean Yellow Mosaic Virus (MYMV) resistant and high yielding greengram varieties was conducted by Krishi Vigyan Kendra, Tamil Nadu Agricultural University, Tirupur in farmers field during kharif 2020. Totally 15 farmers each with one acre were selected in three villages of Pongalur Block in Tirupur district of Tamil Nadu. Critical input viz., seeds of greengram varieties CO 8 and DGG 1 were distributed to the farmers and the varietal performance were assessed along with the existing variety under cultivation VBN 2. The plant physiological parameters viz., plant height, number of nodules / plant and yield attributing parameters such as number of pods / plants, number of branches/plant, Mung bean Yellow Mosaic Virus (MYMV) disease incidence (%), yield (q ha-1) and B:C ratio were recorded. The results revealed that, among the three varieties, Greengram CO 8 was recorded more number of pods (42) and minimum incidence of Mungbean Yellow Mosaic Virus (MYMV) disease incidence of 3.2 per cent resulting in the highest yield of 9.5 q ha-1 followed by DDG 1 with 34 pods/plant, MYMV disease incidence of 7.3 per cent and yield of 8.4 q ha-1 compared to the existing variety VBN 2, which recorded the lowest number of pods (31 Nos.), yield (7.6 q ha-1) with the highest MYMV disease incidence (12.5%). The highest B:C ratio was recorded in greengram variety CO 8 (2.92) which was followed by DDG 1 (2.58). It was concluded that, farmers of Tirupur district in Tamil Nadu were satisfied with cultivation of greengram CO 8 variety due to the lowest disease incidence, higher yield and Benefit Cost Ratio.
RESUMEN
Soil is an important source of available nutrients. Either shortage or surplus of available nutrients in the soil would limit growth of crops. Understanding the spatial variability and distribution patterns of soil available nutrients is essential for soil management with respect to fertilizer application. A total of 111 geo-referenced soil samples were collected on 300 m x 300 m grid at a depth of 0–15 cm, processed and analyzed for pH, EC, soil organic carbon (SOC), available nitrogen (AN), available phosphorus (AP), available potassium (AK), available sulphur (AS), available iron (Fe), available manganese (Mn), available zinc (Zn) and available copper (Cu). Soil properties coefficients of variation (CVs) of soil properties widely varied from low (5.22%) to moderate (49.28%). The geostatistics and geographic information system (GIS) techniques were applied. Ordinary kriging and semivariogram analysis showed differed spatial variability patterns for the studied soil properties with spatial dependence ranged from moderate to strong. The semivariograms for the soil properties were best fitted with spherical model. The range of influence for available N, P, K and S were 268, 287, 497 and 706 m, respectively. The spatial ranges of available Fe, Mn, Zn and Cu were 1050, 1150, 1470, and 1430 m, respectively. The spatial dependence class was strong for EC, SOC, available P, K and was moderate for available N, S, Fe, Mn, Zn and Cu. The available N and P is categorized as low (<280 kg ha-1) and low (<11 kg ha-1) to medium (<22 kg ha-1), respectively were the main limiting factors in crop production. The availability K was categorized as medium (118-280 kg ha-1) to high (> 280 kg ha -1). The soil nutrient maps generated would help to provide precise fertilizer recommendations for sustainable production and environmental conservation.
RESUMEN
Aim: Fungi are increasing in incidence as human pathogens and newer and rarer species are continuously being encountered. Identifying these species from growth on regular culture media may be challenging due to the absence of typical features. An indigenous and cheap medium, similar to the natural substrate of these fungi, was standardised in our laboratory as an aid to species identification in a conventional laboratory setting. Materials and Methods: Ripe banana peel pieces, sterilised in an autoclave at 121°C temperature and 15 lbs pressure for 15 min promoted good growth of hyphae and pycnidia or acervuli in coelomycetes, flabelliform and medusoid fruiting bodies of basidiomycetes and fruit bodies such as cleistothecium in ascomycetes. The growth from the primary isolation medium was taken and inoculated onto the pieces of double‑autoclaved ripe banana peel pieces in a sterile glass Petri dish with some moisture (sprinkles of sterile distilled water). A few sterile coverslips were placed randomly inside the Petri dish for the growing fungus to stick on to it. The plates were kept at room temperature and left undisturbed for 15–20 days. At a time, one coverslip was taken out and placed on a slide with lactophenol cotton blue and focused under the microscope to look for fruit bodies. Results: Lasiodiplodia theobromae, Macrophomina phaseolina, Nigrospora sphaerica, Chaetomium murorum, Nattrassia mangiferae and Schizophyllum commune were identified by characteristic features from growth on banana peel culture. Conclusions: Banana peel culture is a cheap and effective medium resembling the natural substrate of fungi and is useful for promoting characteristic reproductive structures that aid identification.