[Assessment of antibacterial effect of cinnamon on growth of porphyromons gingivalis from chronic periodontitis patients with deep pockets [in vitro]]

Antibiotics are commonly used for controlling the growth of porphyromons gingivalis [P.g] which is one of the most important etiologic factors in the periodontal diseases. Different side effects of synthetics and chemical drugs such as increasing the drug resistancy in the human pathogens have led to study on the herbal antibacterial effect. The aim of this study was to evaluate the antibacterial effect of cinnamon on the growth of porphyromons gingivalis in chronic periodontitis patients with deep pockets. In this experimental study, samples were provided from patients having pockets. After culturing the microorganism and diagnosis of P.g by gram staining and biochemical tests, cinnamon in different concentrations [10, 50, 100, 250, 500, 750 and 1500 mg/ml] with oil solvent were prepared and placed by disks in the cultures medium. Positive controls were amoxicillin, metronidazole, ciprofloxacin, amikacin and gentamycin. Oil was negative control. Then the plates were incubated for 24 hours in 37[0] C and then non-growth halos by disk diffusion method, MIC [Minimum Inhibitory Concentration] and MBC [Minimum Bactericidal Concentration] were determined. Data were analyzed using One-way ANOVA test. The results showed that the cinnamon at the concentration of MIC=750 mg/ml had the inhibitory effects of bacteria and at the concentration of MIC=1500 mg/ml had killing effect. However, this antibacterial effect compared with commonly used antibiotics [amoxicillin, metronidazole], was much weaker [P<0.001]. Cinnamon showed an antimicrobial effect on porphyromonas gingivalis in chronic periodontitis patients with deep pockets

[Anti Streptococcus mutans non fluoride and fluoride containing sealants after adding nano-silver particles]

Since recurrent caries are one of the major causes of failure in resin restorations, the production of antibacterial resin composites was always under investigation. The aim of this study was to evaluate the efficacy of fissure sealants containing nanosilver particles against the Streptococcus mutans. In this experimental study, the antibacterial properties of two sealants [with fluoride [Clinpro 3M] and without fluoride [Concise 3M] was investigated with 0, 0.01, 0.02, 0.03, 0.04, 0.05% w/w after adding nano-silver using direct contact test. Sealants formed on the walls of 500ml micro tube and after curing, they left in contact with bacterial suspension. In periods of 3, 24, 48h, a 10 microl volume of liquid medium was placed in blood agar culture and after 24 h incubation at 37°C, the number of S.mutans colony was counted by colony counter. Data were analyzed using ANOVA and T-test. Results reported sealants with fluoride comparing to non fluoride ones had significant effect on inhibition of S.mutans growth [P<0.001]. The direct contact test demonstrated that by increasing the amount of nano particles, the bacterial growth was significantly diminished [P<0.001]. While sealants with fluoride demonstrated antibacterial effect, sealants with incorporation of higher weight percentage of nanosilver particles, had stronger and more significant antibacterial effect in direct contact test