Proper sex identification through cytogenetic study

The present study is an attempt to specify the genetic sex of a patient who presented with misleading signs of sex. This patient had a feminine distribution of body fat and body hairs, but had no sexual interests. However he had a rudimentary infantile penis, a very small prostate, and nonpalpable testes in the scrotum or the inguinal canal. Serum testosterone was less while serum luteinising hormone and follicle stimulating hormone were higher than the normal level. Cytogenetic investigations proved that he was sex chromatin negative and had 46 chromosomes per cell nucleus with XY as a sex chromosome constitution. Therefore he was classified as a male with hypogonadism of the primary type

rate of sister chromatid exchanges among females from the local environment

Sister chromatid exchanges were studied in cultured lymphocytes from ten normal healthy females. 5-bromodeoxyuridine was added at a final concentration of 30 ug/ml at the beginning of the culture time. Hoechst-Giemsa technique was used in the chromosomal preparations. The mean of SCE frequency was 4.01/metaphase +/- 1.34 and a range of 0-18. The present study revealed the predominance of exchanges between sister chromatids among chromosomes of groups A, B and C