RESUMEN
The presence of the single nucleotide polymorphisms in exon 1 of the mannose-binding lectin 2 (MBL2) gene was evaluated in a sample of 159 patients undergoing coronary artery bypass surgery (71 patients undergoing valve replacement surgery and 300 control subjects) to investigate a possible association between polymorphisms and heart disease with Chlamydia infection. The identification of the alleles B and D was performed using real time polymerase chain reaction (PCR) and of the allele C was accomplished through PCR assays followed by digestion with the restriction enzyme. The comparative analysis of allelic and genotypic frequencies between the three groups did not reveal any significant difference, even when related to previous Chlamydia infection. Variations in the MBL plasma levels were influenced by the presence of polymorphisms, being significantly higher in the group of cardiac patients, but without representing a risk for the disease. The results showed that despite MBL2 gene polymorphisms being associated with the protein plasma levels, the polymorphisms were not enough to predict the development of heart disease, regardless of infection with both species of Chlamydia.
Asunto(s)
Humanos , Masculino , Femenino , Persona de Mediana Edad , Infecciones por Chlamydia/sangre , Infecciones por Chlamydia/genética , Enfermedades de las Válvulas Cardíacas/microbiología , Lectina de Unión a Manosa/sangre , Lectina de Unión a Manosa/genética , Estudios de Casos y Controles , Infecciones por Chlamydia/diagnóstico , Estudios Transversales , Frecuencia de los Genes , Predisposición Genética a la Enfermedad , Genotipo , Enfermedades de las Válvulas Cardíacas/sangre , Enfermedades de las Válvulas Cardíacas/cirugía , Reacción en Cadena de la Polimerasa , Polimorfismo de Nucleótido SimpleRESUMEN
Criança com dois anos e seis meses de idade, apresentando cardiomiopatia dilatada idiopática, classe funcional III-IV (New York Heart Association - NYHA) e indicaçäo de transplante cardíaco, foi submetida à ventriculectomia radioisotópica (Gated Blood Pool) revelaram fraçäo de ejeçäo de ventrículo esquerdo (FEVE) de 13 'por cento' e 20 'por cento', respectivamente. Após a cirurgia, que consistiu na remoçäo de uma parte do músculo da parede lateral do ventrículo esquerdo, a FEVE foi para 50 'por cento' pelo ECO e 30 'por cento' pela ventriculografia radioisotópica. A criança recebeu alta hospitalar no 16§ pós-operatório em classe funcional I (NYHA).