RESUMEN
Objective To establish a HPLC method for the simultaneous determination of quercetin and mignonette in Gnaphalium affine. Methods The determination was performed on Diamonsil C18 (2) column (4.6 mm×250 mm, 5 μm), mobile phase was methanol-0.4％ phosphoric acid solution (42:58), detection wavelength was set at 360 nm, column temperature was 30℃, flow rate was 1 mL · min-1 and the injection volume was 20 μL. Results The linear rages of quercetin and mignonette were 3.588-35.880 μg · mL-1 (R2=0.999 6) and 1.294-12.940 μg · mL-1 (R2=0.999 4) respectively. Their average recoveries (n=6) were 99.51％ (RSD=0.31％) and 99.74％ (RSD=2.28％) respectively.Quercetin Content was 0.39-0.58 mg·g-1, mignonette content was 0.17-0.27 mg·g-1.Conclusion The method is simple, accurate, reliable and repeatable, thus it can be used for the quality control of Gnaphalium affine.