Study of Feasibility, Validity, Reliability, and Norm-Finding of Scale of Social Styles in Employees of Tehran Regional Electricity Company

In this study, the feasibility, validity, reliability, and normality of social style scales are investigated among the employees of RegionalElectricity Company of Tehran. In this exploratory study, a descriptive method was used. The sample size was 350 people (194 males and156 females), which were selected using a random sampling method. The social style scale pointed based on a 4-point Likert scalecontaining 30 items. The reliability of the questionnaire was determined using Cronbach's alpha and no item was excluded from thequestionnaire. To test the construct validity of the instrument, the principal component analysis (PCA) method was used. To test thestructure of the scale of derived factors, the Bartlett test was used by means of tilt rotation and 6 factors were derived including driving,expressive, analytical, amiable, compassion, and lawful

Investigation of mutations in exons 12-15 MYH7 gene in hypertrophic cardiomyopathie patients using PCR-SSCP technique

Hypertrophic cardiomyopathy [HCM] is the most common kind of Mendelian inherited heart disease, affects 0.2% of the global population. HCM is also the most common cause of sudden cardiac death in individuals younger than 35 years old. To date more than 900 individual mutations has been identified in over 20 genes, such as MYH7, MYBPC3, and TNNT2. Interestingly, most of these genes encode sarcomeric proteins. In the present study, we investigated the possible presence of mutation in exons 12-15 MYH7 gene, which has already been reported to accommodate some mutations, in 30 patients with HCM in Chaharmahal va Bakhtiyari province. DNA was extracted using standard phenol-chloroform method and then was used for amplification and gel electroploresis by PCR-SSCP procedure. Finally, the suspected cases were selected for the direct sequencing and the results were analyzed using chromas software. There is no mutation in these exons, but two polymorphisms including: 5811 C>T and 5845 G> were found in the exon 12 of 1 and 5 separate patients, respectively. In this study with respect to none amino acid codon changes arisen from these polymorphisms, we concluded that mutations in these exons of MYH7 gene have a very low contribution in patients in this province and this is necessary to study other exons for better assessment